Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino-cho, Nobeoka, Miyazaki, 882-8508, Japan.
Department of Pathology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
J Nat Med. 2021 Sep;75(4):975-984. doi: 10.1007/s11418-021-01518-w. Epub 2021 May 4.
Brazilian propolis (AF-08) is a dietary supplement containing a variety of flavonoids. It is used worldwide as a folk medicine. Flavonoids and a diet of fruits and vegetables containing them have been shown to reduce the risk of cardiovascular diseases (CVDs). Most of CVDs are caused by arterial thrombus formation. A thrombus is formed by the interaction between adhesion and aggregation of platelets to damaged blood vessels and blood coagulation consisting of extrisic and intrinsic pathways. Platelet aggregation and blood coagulation are closely linked to thrombosis. Therefore, we evaluated the effectiveness of AF-08 or its component flavonoids against thrombosis by examining their inhibition of platelet aggregation and blood coagulation. Human platelet-rich plasma was incubated with serial dilutions of AF-08 for 10 min to assess its inhibitory effect on platelet aggregation caused by collagen. The inhibitory effect of AF-08 on blood coagulation was evaluated by the prothrombin time (PT) and activated partial thromboplastin time (APTT), which reflect the coagulation function of extrinsic and intrinsic pathways, respectively. AF-08 significantly inhibited collagen-induced platelet aggregation but not PT and APTT, indicating that AF-08 inhibited platelet aggregation but not blood coagulation. Among three flavonoids contained in AF-08, apigenin and chrysin obviously inhibited platelet aggregation but the inhibitory effect of kaempferol was less effective. The three flavonoids did not affect PT and APTT. The inhibitory activity of AF-08 on human platelet aggregation without affecting blood coagulation was suggested to be partially due to apigenin and chrysin. AF-08 may be effective in suppressing platelet-based arterial thrombus formation and reducing the risk of CVDs.
巴西蜂胶（AF-08）是一种含有多种类黄酮的膳食补充剂。它在全球范围内被用作民间药物。类黄酮和富含类黄酮的水果和蔬菜饮食已被证明可降低心血管疾病（CVDs）的风险。大多数 CVDs 是由动脉血栓形成引起的。血栓是由血小板与受损血管的粘附和聚集以及由外源性和内源性途径组成的血液凝固之间的相互作用形成的。血小板聚集和血液凝固与血栓形成密切相关。因此，我们通过检查其对血小板聚集和血液凝固的抑制作用，评估了 AF-08 或其成分类黄酮对血栓形成的有效性。将富含人类血小板的血浆与 AF-08 的系列稀释液孵育 10 分钟，以评估其对胶原诱导的血小板聚集的抑制作用。通过凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）评估 AF-08 对血液凝固的抑制作用，这分别反映了外源性和内源性途径的凝血功能。AF-08 显著抑制胶原诱导的血小板聚集，但不抑制 PT 和 APTT，表明 AF-08 抑制血小板聚集但不抑制血液凝固。AF-08 中包含的三种类黄酮中，芹菜素和白杨素明显抑制血小板聚集，但山柰酚的抑制作用效果较差。这三种类黄酮不影响 PT 和 APTT。AF-08 对人类血小板聚集的抑制作用而不影响血液凝固的活性部分归因于芹菜素和白杨素。AF-08 可能有效抑制基于血小板的动脉血栓形成并降低 CVDs 的风险。
