Inoue T, Tashima T, Nishio S, Takeshita I, Iwaki T, Fukui M
Department of Neurosurgery, Faculty of Medicine, Kyushu University, Japan.
Acta Neurochir (Wien). 1988;91(1-2):67-72. doi: 10.1007/BF01400531.
Vascular permeability and proliferative activity of ethylnitrosourea (ENU)-induced rat brain tumours were studied by intravenous injection of Evans blue dye (EB) and by bromodeoxyuridine (BrdU) uptake examinations. Tumours induced by ENU showed various histologial types, and they were oligodendrogliomas, mixed oligo-astrocytomas, mixed oligo-ependymomas, astrocytomas, anaplastic astrocytomas, polymorphic gliomas, and ependymomas. The labelling indexes (LIs: the ratio of BrdU-labelled cells to total cells) of tumour and vascular component cells in the tumour were high in anaplastic astrocytomas, polymorphic gliomas and ependymomas, but low in oligodendrogliomas. EB stained anaplastic astrocytomas, polymorphic gliomas and ependymomas deeply, but did not penetrate oligodendrogliomas. In mixed gliomas, EB staining and the LIs of tumour cells were not uniform. After intracarotid infusion of hyperosmolar mannitol into tumour-bearing rats, tumour staining with EB and the LIs of tumour cells were not increased, whereas the penetration of EB into the normal brain was drastically increased. Therefore it is not likely that the delivery of chemotherapeutic drugs to the tumour could be increased by intracarotid infusion of hyperosmolar mannitol. Our data suggest that the vascular permeability of tumour vessels is highly correlated with the high proliferative activity of tumour and its vascular cells.
通过静脉注射伊文思蓝染料（EB）以及进行溴脱氧尿苷（BrdU）摄取检查，研究了乙基亚硝基脲（ENU）诱导的大鼠脑肿瘤的血管通透性和增殖活性。ENU诱导的肿瘤呈现出多种组织学类型，包括少突胶质细胞瘤、少突-星形细胞瘤混合型、少突-室管膜瘤混合型、星形细胞瘤、间变性星形细胞瘤、多形性胶质瘤和室管膜瘤。间变性星形细胞瘤、多形性胶质瘤和室管膜瘤中肿瘤及肿瘤血管成分细胞的标记指数（LIs：BrdU标记细胞与总细胞的比率）较高，而少突胶质细胞瘤中的标记指数较低。EB可使间变性星形细胞瘤、多形性胶质瘤和室管膜瘤染色较深，但不能穿透少突胶质细胞瘤。在混合性胶质瘤中，EB染色和肿瘤细胞的LIs并不一致。给荷瘤大鼠颈内动脉输注高渗甘露醇后，肿瘤的EB染色和肿瘤细胞的LIs并未增加，而EB向正常脑组织的渗透却显著增加。因此，通过颈内动脉输注高渗甘露醇来增加化疗药物向肿瘤的递送不太可能。我们的数据表明，肿瘤血管的通透性与肿瘤及其血管细胞的高增殖活性高度相关。
