Groothuis D R, Fischer J M, Lapin G, Bigner D D, Vick N A
J Neuropathol Exp Neurol. 1982 Mar;41(2):164-85. doi: 10.1097/00005072-198203000-00006.
The permeability of different brain tumor models to horseradish peroxidase (HRP) was examined by determining the fraction of tumor that contained HRP after intravenous administration. The intracerebral tumor models studied were Avian Sarcoma Virus (ASV)-induced tumors and tumors from transplanted RG-2, S69-C1-5, and 9L cell lines. The average fraction of RG-2 tumors permeable to HRP was .95; of S69-C1-5 tumors, .699; of ASV-induced tumors. .63; and of 9L tumors, .52. Except for the RG-2 tumors, there was considerable regional variation in HRP permeability, which was most marked in the ASV-induced tumors. In ASV-induced tumors, HRP permeability did not correlate with tumor histological classification, size, or anatomic location within the brain. The subcutaneous tumor models studied were RG-2-, S69-C1-5, and 9L-transplanted tumors in rats, and human glioblastoma cell lines transplanted into nude mice. All were completely permeable to HRP. These results indicate that significant differences in permeability to HRP exist among brain tumor models when the tumors are intracerebral, and that all subcutaneous tumors from transplanted glial cell lines are completely permeable to HRP. These variables must be considered in future studies of permeability in experimental brain tumors. Care must be exercised in extrapolating results about permeability from one brain tumor model to another.
通过静脉注射后测定含有辣根过氧化物酶（HRP）的肿瘤部分，研究了不同脑肿瘤模型对HRP的通透性。所研究的脑内肿瘤模型包括禽肉瘤病毒（ASV）诱导的肿瘤以及移植的RG-2、S69-C1-5和9L细胞系产生的肿瘤。RG-2肿瘤对HRP通透的平均比例为0.95；S69-C1-5肿瘤为0.699；ASV诱导的肿瘤为0.63；9L肿瘤为0.52。除RG-2肿瘤外，HRP通透性存在显著的区域差异，在ASV诱导的肿瘤中最为明显。在ASV诱导的肿瘤中，HRP通透性与肿瘤组织学分类、大小或脑内解剖位置无关。所研究的皮下肿瘤模型包括大鼠体内移植的RG-2、S69-C1-5和9L肿瘤，以及移植到裸鼠体内的人胶质母细胞瘤细胞系。所有这些对HRP均完全通透。这些结果表明，当肿瘤位于脑内时，不同脑肿瘤模型对HRP的通透性存在显著差异，并且所有移植胶质细胞系产生的皮下肿瘤对HRP均完全通透。在未来实验性脑肿瘤通透性研究中必须考虑这些变量。在将一个脑肿瘤模型的通透性结果外推至另一个模型时必须谨慎。
