State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China; Jiaxing Tongji Institute for Environment, Jiaxing, Zhejiang, 314051, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, PR China; Jiaxing Tongji Institute for Environment, Jiaxing, Zhejiang, 314051, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, PR China.
Chemosphere. 2021 Oct;280:130666. doi: 10.1016/j.chemosphere.2021.130666. Epub 2021 Apr 27.
Short-chained perfluorobutane sulfonate (PFBS, four-carbon) and perfluorohexane sulfonate (PFHxS, six-carbon) are widely employed to substitute long-chained per- and poly-fluoroalkyl substances (PFASs). Recent studies showed the potential persistence of PFBS and PFHxS, and also reported their correlation with obesity. However, the long-term outcome and underlying mechanisms remained poorly understood. Presently, the effects of PFBS and PFHxS were studied on C. elegans with multi- and trans-generational experiments. The multi-generational effects were measured in continuous four generational exposure (i.e., F1 to F4). Results showed that PFBS did not stimulate the fat content in F1 but in F4 with continuous but different disturbances on the lipid metabolism and the insulin and insulin-like (IIS) pathway. PFHxS stimulated the fat content in F1 and F4 with similar disturbances on the lipid metabolism and IIS pathway. The trans-generational results showed that the effects of PFBS and PFHxS on the lipid metabolism and IIS pathway were not totally recovered in the offspring of F1 (i.e., T1-T3) and F4 (i.e., T1'-T3') which were not continuously exposed. PFHxS showed a common pattern to up-regulate daf-7 in both multi- and trans-generational effects. The long-term consequences of the short-chained PFASs substitutes should be concerned and epigenetic regulations should be considered in future mechanism studies.
短链全氟丁烷磺酸（PFBS，四碳）和全氟己烷磺酸（PFHxS，六碳）被广泛用于替代长链全氟和多氟烷基物质（PFASs）。最近的研究表明，PFBS 和 PFHxS 具有潜在的持久性，并且与肥胖有关。然而，其长期后果和潜在机制仍知之甚少。目前，通过多代和跨代实验研究了 PFBS 和 PFHxS 对秀丽隐杆线虫的影响。多代实验的影响在连续四代暴露（即 F1 到 F4）中进行了测量。结果表明，PFBS 虽然在 F4 代中刺激了脂肪含量的增加，但在 F4 代中对脂代谢和胰岛素及胰岛素样（IIS）通路的干扰是连续但不同的。PFHxS 在 F1 和 F4 代中刺激了脂肪含量的增加，对脂代谢和 IIS 通路的干扰相似。跨代实验结果表明，PFBS 和 PFHxS 对脂代谢和 IIS 通路的影响在未连续暴露的 F1（即 T1-T3）和 F4（即 T1'-T3'）后代中并未完全恢复。PFHxS 在多代和跨代实验中均表现出上调 daf-7 的共同模式。应该关注短链 PFASs 替代品的长期后果，并在未来的机制研究中考虑表观遗传调控。
