Chen Chuying, Chen Jinyin, Wan Chunpeng
Jiangxi Key Laboratory for Postharvest Technology and Non-destructive Testing of Fruits & Vegetables/Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruit and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Pingxiang University, Pingxiang 337055, China.
Microorganisms. 2020 Apr 8;8(4):536. doi: 10.3390/microorganisms8040536.
The current study aimed to examine the in vitro and in vivo antifungal potential of pinocembrin-7-glucoside (P7G). P7G is an antifungal flavanone glycoside isolated from Vahl. fruit against , a causative pathogen of blue mold disease in citrus fruit, and this study elucidates its possible action mechanism. P7G had a prominent mycelial growth inhibitory activity against , with an observed half maximal effective concentration, minimum inhibitory concentration and minimum fungicidal concentration of 0.08, 0.2, and 0.8 g/L, respectively. The data from the in vivo test show that P7G significantly reduced blue mold symptoms and disease development of in artificially inoculated "Newhall" navel orange. Compared to the control, increases in the cell membrane permeability of supernatant and decreases in the intracellular constituent (e.g., soluble protein, reducing sugar, and total lipid) contents of mycelia were identified, supporting scanning electron microscopy and transmission electron microscopy observations. Furthermore, a marked decline in both chitin and glucanase contents of mycelia treated with P7G was induced by increasing its related degrading enzyme activities, suggesting that the cell wall structure was destroyed. The current study indicated that P7G may be a novel alternative for reducing blue mold by suppressing mycelial growth of via a cell membrane/wall-targeting mechanism.
本研究旨在考察松属素-7-葡萄糖苷(P7G)的体外和体内抗真菌潜力。P7G是一种从[植物名称未完整给出]果实中分离得到的抗真菌黄烷酮糖苷,对柑橘果实青霉病的致病病原体[病原体名称未完整给出]具有活性,本研究阐明了其可能的作用机制。P7G对[病原体名称未完整给出]具有显著的菌丝生长抑制活性,观察到的半数最大有效浓度、最小抑菌浓度和最小杀菌浓度分别为0.08、0.2和0.8 g/L。体内试验数据表明,P7G显著减轻了人工接种“纽荷尔”脐橙中[病原体名称未完整给出]的青霉症状和病害发展。与对照相比,发现[病原体名称未完整给出]上清液的细胞膜通透性增加,其菌丝体的细胞内成分(如可溶性蛋白、还原糖和总脂质)含量降低,这支持了扫描电子显微镜和透射电子显微镜的观察结果。此外,用P7G处理的[病原体名称未完整给出]菌丝体中几丁质和葡聚糖酶含量显著下降,同时其相关降解酶活性增加,表明细胞壁结构被破坏。本研究表明,P7G可能是一种通过细胞膜/细胞壁靶向机制抑制[病原体名称未完整给出]菌丝生长来减少青霉病的新型替代物。