Platt David C, Rink Jonathan, Braich Kamaljit, McLauchlan Craig C, Jones Marjorie A
Department of Chemistry, Illinois State University, Normal, IL 61790-4160, USA.
Brain Sci. 2021 Apr 30;11(5):588. doi: 10.3390/brainsci11050588.
The enzyme, 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) has been known for over fifty years. Nevertheless, the roles this membrane-bound enzyme play have yet to be described completely. Recently, there has been renewed interest in the study of this enzyme due to studies that suggest that CNPase plays a role in the mediation of cellular inflammatory responses in renal and nervous system tissues. Also, this enzyme, found in oligodendrocytes of the nervous system, has been reported to participate in significant regulatory changes associated with age which may be involved in age-related CNS degeneration. Consequently, development of CNPase inhibitors is of interest and should aid in the study of this, as yet, poorly understood enzyme. In this work we utilized a spectrophotometric enzyme assay to determine the effect a panel of organo-vanadium complexes had on isolated hamster myelin CNPase activity. Our group has now identified several potent in vitro CNPase inhibitors that could prove useful in clarifying the important roles of this enzyme.
2'-3'-环核苷酸3'-磷酸二酯酶（CNPase）这种酶已为人所知五十多年了。然而，这种膜结合酶所起的作用尚未被完全描述。最近，由于一些研究表明CNPase在肾脏和神经系统组织的细胞炎症反应介导中发挥作用，对该酶的研究重新引起了人们的兴趣。此外，这种在神经系统少突胶质细胞中发现的酶，据报道参与了与年龄相关的显著调节变化，这可能与年龄相关的中枢神经系统退化有关。因此，开发CNPase抑制剂很有意义，并且应该有助于对这种尚未被充分理解的酶的研究。在这项工作中，我们利用分光光度酶分析法来确定一组有机钒配合物对分离的仓鼠髓磷脂CNPase活性的影响。我们小组现已鉴定出几种有效的体外CNPase抑制剂，这些抑制剂可能有助于阐明这种酶的重要作用。
