来自法属西印度群岛（马提尼克岛）临床和环境分离株中的唑类耐药性
Azole Resistance in Clinical and Environmental s Isolates from the French West Indies (Martinique).
作者信息
Monpierre Lorra, Desbois-Nogard Nicole, Valsecchi Isabel, Bajal Marielle, Angebault Cécile, Miossec Charline, Botterel Françoise, Dannaoui Éric
机构信息
Unité de Parasitologie-Mycologie, Département Prévention, Diagnostic, Traitement des infections, CHU Henri Mondor, Assistance Publique des Hôpitaux de Paris (AP-HP), 94140 Créteil, France.
EA DYNAMiC 7380, Faculté de Santé, Univ Paris-Est Créteil (UPEC), Créteil, France-École Nationale Vétérinaire d'Alfort (ENVA), USC Anses, 94700 Maison-Alfort, France.
出版信息
J Fungi (Basel). 2021 Apr 30;7(5):355. doi: 10.3390/jof7050355.
The emergence of azole resistant spp., especially , has been described in several countries around the world with varying prevalence depending on the country. To our knowledge, azole resistance in spp. has not been reported in the West Indies yet. In this study, we investigated the antifungal susceptibility of clinical and environmental isolates of spp. from Martinique, and the potential resistance mechanisms associated with mutations in gene. Overall, 208 isolates were recovered from clinical samples ( = 45) and environmental soil samples ( = 163). They were screened for resistance to azole drugs using selective culture media. The Minimum Inhibitory Concentrations (MIC) towards voriconazole, itraconazole, posaconazole and isavuconazole, as shown by the resistant isolates, were determined using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) microdilution broth method. Eight isolates (, = 6 and , = 2) had high MIC for at least one azole drug. The sequencing of gene revealed the mutations G54R and TR34/L98H in two clinical isolates. Our study showed for the first time the presence of azole resistance in and isolates in the French West Indies.
唑类耐药的某菌属（原文此处未明确写出菌属名称）的出现，尤其是该菌属（原文此处未明确写出菌属名称），在世界上几个国家都有描述，其流行率因国家而异。据我们所知，西印度群岛尚未报道过该菌属（原文此处未明确写出菌属名称）的唑类耐药情况。在本研究中，我们调查了来自马提尼克岛的该菌属（原文此处未明确写出菌属名称）临床和环境分离株的抗真菌药敏性，以及与某基因（原文此处未明确写出基因名称）突变相关的潜在耐药机制。总体而言，从临床样本（n = 45）和环境土壤样本（n = 163）中分离出208株该菌属（原文此处未明确写出菌属名称）菌株。使用选择性培养基筛选它们对唑类药物的耐药性。采用欧洲抗菌药物敏感性试验委员会（EUCAST）微量稀释肉汤法测定耐药菌株对伏立康唑、伊曲康唑、泊沙康唑和艾沙康唑的最低抑菌浓度（MIC）。8株菌株（某菌属（原文此处未明确写出菌属名称），n = 6和某菌属（原文此处未明确写出菌属名称），n = 2）对至少一种唑类药物的MIC较高。某基因（原文此处未明确写出基因名称）测序显示，两株该菌属（原文此处未明确写出菌属名称）临床分离株存在G54R和TR34/L98H突变。我们的研究首次表明，法属西印度群岛的该菌属（原文此处未明确写出菌属名称）和某菌属（原文此处未明确写出菌属名称）分离株中存在唑类耐药情况。
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