Zerbinati Nicola, Sommatis Sabrina, Maccario Cristina, Di Francesco Serena, Capillo Maria Chiara, Rauso Raffaele, Herrera Martha, Bencini Pier Luca, Guida Stefania, Mocchi Roberto
Department of Medicine and Surgery, University of Insubria, 21100 Varese, Italy.
Department of Molecular Medicine, UB-CARE S.r.l.-Spin-Off University of Pavia, 27100 Pavia, Italy.
Life (Basel). 2021 Apr 29;11(5):406. doi: 10.3390/life11050406.
Skin ageing has many manifestations such as wrinkles, dryness, hyperpigmentation, and uneven skin tone. Extrinsic and intrinsic factors, especially solar ultraviolet light (UVB), contribute to skin ageing; its main features are brown spots, alterations in melanin pigmentation, and a decrease in collagen and hyaluronic acid linked to oxidative stress. Several studies showed that topical products containing ingredients with antioxidant activity can reduce oxidative damage; to provide a maximum anti-ageing effect to the skin, topical products can combine various ingredients. C-SHOT SERUM contains a combination of two molecules with a proven anti-ageing activity: a high percentage (30%) of a more stable vitamin C derivative, 3--ethyl-l-ascorbic acid, and lactic acid (1%). The product showed a high biocompatibility, assessed through an MTT assay on keratinocytes and on Reconstructed Human Epidermis (RHE, SkinEthic); the anti-ageing activity was demonstrated on human dermal fibroblasts and keratinocytes by a statistically significant increase in collagen production and a reduction of a UVB-induced DNA damage marker (-H2AX histone), indicating DNA protection. Moreover, a depigmenting activity, shown by a highly significant decrease in melanin content on treated Reconstructed Human Pigmented Epidermis (RHPE), was assessed. According to the data of our study, the tested product contrasts the effect of skin ageing and irregular pigmentation due to the physiological decline of the skin.
皮肤老化有许多表现，如皱纹、干燥、色素沉着和肤色不均。外在和内在因素，尤其是太阳紫外线（UVB），会导致皮肤老化；其主要特征是褐色斑点、黑色素沉着改变以及与氧化应激相关的胶原蛋白和透明质酸减少。多项研究表明，含有具有抗氧化活性成分的外用产品可以减少氧化损伤；为了给皮肤提供最大的抗老化效果，外用产品可以组合多种成分。C-SHOT精华液含有两种具有已证实抗老化活性的分子组合：高百分比（30%）的更稳定的维生素C衍生物3-乙基-L-抗坏血酸和乳酸（1%）。通过对角质形成细胞和重建人表皮（RHE，SkinEthic）进行MTT试验评估，该产品显示出高生物相容性；在人真皮成纤维细胞和角质形成细胞上，通过胶原蛋白产量的统计学显著增加和UVB诱导的DNA损伤标志物（-H2AX组蛋白）的减少证明了其抗老化活性，表明具有DNA保护作用。此外，在处理过的重建人色素沉着表皮（RHPE）上，通过黑色素含量的高度显著降低显示出了其美白活性。根据我们的研究数据，该测试产品可对抗因皮肤生理衰退导致的皮肤老化和色素沉着不均的影响。
