Yilmaz Caner, Ersanli Selim, Karabagli Murat, Olgac Vakur, Bolukbasi Balcioglu Nilufer
Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul 34093, Turkey.
Department of Surgery, Faculty of Veterinary, Istanbul University Cerrahpasa, Istanbul 34098, Turkey.
Medicina (Kaunas). 2021 Apr 29;57(5):430. doi: 10.3390/medicina57050430.
: Guided bone regeneration (GBR) surgeries are used for dental implant placements with insufficient bone volume. Biomaterials used in GBR are expected to produce sufficient volume and quality of bone swiftly. This study aims to histologically evaluate the effectiveness of the use of Hyalonect membranes alone or with autogenous grafts in intraosseous defects. : This study is an experimental study on sheep. Surgeries were performed under general anesthesia in accordance with ethical rules. Five 10 mm defects were surgically created in each ilium of six sheep. One defect was left empty in each ilium (group ED). The defects in the experimental group were covered with Hyalonect membrane while unfilled (group HY) or after being filled with autogenous bone grafts (ABG) (group G+HY). In the control group, the defects were either covered with collagen membrane while unfilled (group CM) or after being filled with the ABG group (G+CM). The sheep were histologically and histomorphometrically evaluated after being postoperatively sacrificed in the third and sixth week (three animals in each interval). : All animals completed the study without any complications. No difference was found between groups in the third and sixth weeks regarding the inflammation, necrosis, and fibrosis scores. The G+CM (52.83 ± 3.06) group was observed to have a significantly higher new bone formation rate than all the other groups in the third week, followed by the G+HY group (46.33 ± 2.25). Similar values were found for HY and CM groups (35.67 ± 4.55 ve 40.00 ± 3.41, respectively, = 0.185), while the lowest values were observed to be in group ED (19.67 ± 2.73). The highest new bone formation was observed in group G+CM (82.33 ± 4.08) in the sixth week. There was no difference in new bone formation rates between groups G+CM, G+HY (77.17 ± 3.49, = 0.206), and CM (76.50 ± 2.43, = 0.118). The insignificant difference was found ED group and group HY (55.83 ± 4.92, 73.50 ± 3.27, respectively, = 0.09). The residual graft amount in the G+CM group was found to be statistically significant at 3 weeks ( = 0.0001), compared to the G+HY group, and insignificantly higher at the 6th week ( = 0.4). : In this study, close values were observed between G+HY and G+CM groups. Further experimental and clinical studies with different graft materials are required to evaluate the effectiveness of HY in GBR.
引导骨再生（GBR）手术用于骨量不足的牙种植体植入。GBR中使用的生物材料有望迅速产生足够的骨量和骨质量。本研究旨在通过组织学评估单独使用透明质酸膜或与自体移植物联合使用在骨内缺损中的有效性。：本研究是一项针对绵羊的实验研究。手术在全身麻醉下按照伦理规则进行。在六只绵羊的每只髂骨上手术制造五个10毫米的缺损。每只髂骨留一个缺损为空（ED组）。实验组的缺损在未填充时用透明质酸膜覆盖（HY组）或在填充自体骨移植（ABG）后覆盖（G+HY组）。在对照组中，缺损在未填充时用胶原膜覆盖（CM组）或在填充ABG后覆盖（G+CM组）。绵羊在术后第三周和第六周处死进行组织学和组织形态计量学评估（每个时间段三只动物）。：所有动物均无并发症地完成了研究。在第三周和第六周，各组在炎症、坏死和纤维化评分方面没有差异。观察到G+CM组（52.83±3.06）在第三周的新骨形成率显著高于所有其他组，其次是G+HY组（46.33±2.25）。HY组和CM组的值相似（分别为35.67±4.55和40.00±3.41，P=0.185），而最低值出现在ED组（19.67±2.73）。在第六周，G+CM组的新骨形成最高（82.33±4.08）。G+CM组、G+HY组（77.17±3.49，P=0.206）和CM组（76.50±2.43，P=0.118）之间的新骨形成率没有差异。ED组和HY组之间差异不显著（分别为55.83±4.92和73.50±3.27，P=0.09）。发现G+CM组在3周时的剩余移植物量与G+HY组相比具有统计学意义（P=0.0001），在第6周时略高但无统计学意义（P=0.4）。：在本研究中，G+HY组和G+CM组之间观察到相近的值。需要进一步进行不同移植材料的实验和临床研究来评估透明质酸膜在GBR中的有效性。
