Ondur Esra, Bolukbasi Balcioglu Nilufer, Soluk Tekkesin Merva, Guzel Ozlem, Ersanli Selim
Department of Oral Implantology, Faculty of Dentistry, Istanbul University, Istanbul 34093, Turkey.
Department of Tumor Pathology, Institute of Oncology, Istanbul University, Istanbul 34093, Turkey.
Materials (Basel). 2020 Apr 4;13(7):1695. doi: 10.3390/ma13071695.
Bone defects lead to aesthetic and functional losses, causing dental rehabilitation to be more difficult. The objective of this work is to histologically assess the hard tissue healing of bone defects filled with platelet-rich fibrin (PRF) alone or as an adjuvant for mixing with and covering anorganic bovine bone (ABB), compared to ABB covered with a resorbable collagen membrane (CM). This study was designed as a crossover animal study. Four 5-mm tibia defects, 5 mm apart from each other, were surgically created on the tibias of 6 sheep. The defects were randomly filled with ABB + CM; PRF alone; ABB+PRF; or were left empty. The animals were euthanized on days 10, 20, and 40 post-operatively. No group showed any signs of bone necrosis. Inflammation was observed in 2 control and 3 test defects with no statistically significant difference between groups at each time point. The ABB + CM and ABB + PRF groups experienced the highest bone regeneration ratios. No differences between the empty-defect and PRF groups were observed in regard to bone regeneration. No statistical difference was observed between the ABB+PRF and ABB + CM groups in regard to bone regeneration and the amount of residual graft material at each time point. The use of PRF should be preferred due to its autogenous origin, low cost, and ease of use.
骨缺损会导致美观和功能丧失，使牙齿修复更加困难。本研究的目的是通过组织学方法评估单独使用富含血小板纤维蛋白（PRF）或作为辅助材料与无机牛骨（ABB）混合并覆盖时，骨缺损的硬组织愈合情况，并与覆盖可吸收胶原膜（CM）的ABB进行比较。本研究设计为交叉动物研究。在6只绵羊的胫骨上手术制造4个彼此相距5毫米的5毫米胫骨缺损。缺损被随机填充为ABB + CM；单独的PRF；ABB+PRF；或保持空置。在术后第10、20和40天对动物实施安乐死。没有一组出现任何骨坏死迹象。在2个对照缺损和3个试验缺损中观察到炎症，各时间点组间无统计学显著差异。ABB + CM组和ABB + PRF组的骨再生率最高。在骨再生方面，空置缺损组和PRF组之间未观察到差异。在每个时间点，ABB+PRF组和ABB + CM组在骨再生和残余移植材料量方面均未观察到统计学差异。由于PRF的自体来源、低成本和易用性，应优先使用PRF。
