Doganay Ozge, Tugrul Mehmet, Olgac Vakur, Atalay Belir
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University.
Department of Anesthesiology, Istanbul Faculty of Medicine.
J Craniofac Surg. 2019 Jun;30(4):1308-1313. doi: 10.1097/SCS.0000000000005428.
The aim of this study was to investigate the effects of Bioglue as a mechanical barrier with or without biphasic calcium phosphate (BCP) in a rat tibia model. Sixty Sprague Dawley male rats weighing 250 ± 20 g and 10 to 12 weeks of age were studied. Unicortical defects were created on the right tibia of all rats. Subjects were randomly divided into 3 groups. BioGlue group (24 rats); BioGlue alone, Graft group (24 rats); BioGlue + BCP and Control group; unfilled and uncovered (12 rats). Animals were euthanized at 7th, 21st, and 45th days postoperatively for histological and histomorphometric analyses. BioGlue material exhibited no adverse effects until the end of observation period. Bone-healing scores did not differ statistically between Control and BioGlue group, but found to be lower in Graft group on 21st and 45th days, (P < 0.001 and P < 0.01 on the 21st day and P < 0.01 and P < 0.05 on the 45th day, respectively). New bone formation in Graft group was found to be statistically different from Control group on the 7th and 21st days (P < 0.01 and P < 0.05 respectively), whereas no statistical difference was observed between BioGlue and Control group at all times. The present analysis indicates that BioGlue functioned well as a mechanical barrier allowing new bone formation. No additional benefit of combination treatment was detected in this study design and BCP did not offer any advantage for bone regeneration, thus it can serve as only a space maintainer.
本研究旨在探讨在大鼠胫骨模型中，生物胶作为机械屏障单独使用或与双相磷酸钙（BCP）联合使用的效果。研究选用了60只体重250±20克、10至12周龄的雄性Sprague Dawley大鼠。在所有大鼠的右侧胫骨上制造单皮质缺损。将实验对象随机分为3组。生物胶组（24只大鼠），仅使用生物胶；移植组（24只大鼠），生物胶+BCP；对照组，不填充且不覆盖（12只大鼠）。在术后第7天、21天和45天对动物实施安乐死，进行组织学和组织形态计量学分析。在观察期结束前，生物胶材料未表现出任何不良反应。对照组和生物胶组之间的骨愈合评分无统计学差异，但在第21天和45天发现移植组的骨愈合评分较低（第21天P<0.001和P<0.01，第45天P<0.01和P<0.05）。发现移植组在第7天和21天的新骨形成与对照组有统计学差异（分别为P<0.01和P<0.05），而生物胶组和对照组在所有时间点均未观察到统计学差异。目前的分析表明，生物胶作为允许新骨形成的机械屏障发挥了良好作用。在本研究设计中未检测到联合治疗的额外益处，BCP对骨再生未提供任何优势，因此它仅可作为空间维持物。
