College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Xianyang 712100, China.
Medical College, Qinghai University, Xining 810001, China.
Genes (Basel). 2021 Apr 29;12(5):671. doi: 10.3390/genes12050671.
MicroRNAs play an essential role in mammary gland development, and involution is a factor that limits lactation. Chi-miR-8516 is one of the validated microRNAs that regulates the expression of and , which surge during the involution of the mammary gland. This study aims to explore the direct or indirect regulation of and by chi-miR-8516 and the regulation of chi-miR-8516 by circ-140. In goat mammary epithelial cells, we found that chi-miR-8516 takes circ-140 as a sponge and regulates expression by targeting and promoting the phosphorylation of MAPK. The examination of αs1-/β-casein and lipid showed the modulation of the circ-140/chi-miR-8516/- axis in casein secretion and lipid formation, which was regulated by the phosphorylation of mTOR and STAT5. This study illustrates an axis that regulates the synthesis of milk components, and explores the pathways in which the axis participates.
微小 RNA 在乳腺发育中发挥重要作用，而 involution 是限制泌乳的一个因素。Chi-miR-8516 是一种已被验证的调节 和 的表达的 microRNA，它们在乳腺 involution 期间激增。本研究旨在探索 chi-miR-8516 对 和 的直接或间接调节，以及 circ-140 对 chi-miR-8516 的调节。在山羊乳腺上皮细胞中，我们发现 chi-miR-8516 以 circ-140 为海绵，并通过靶向 和促进 MAPK 磷酸化来调节 表达。αs1-/β-casein 和脂质的检测表明，circ-140/chi-miR-8516/-轴在酪蛋白分泌和脂质形成中的调节受到 mTOR 和 STAT5 磷酸化的调节。本研究说明了一个调节乳成分合成的轴，并探索了该轴参与的途径。
