Zhang Yue, Liu Jidan, Niu Guanglin, Wu Qiong, Cao Binyun
College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China; Department of Oncology Pathology, Karolinska Institutet, 17164, Stockholm, Sweden; School of Life Sciences, Technical University of Munich, 85354, Freising, Germany.
College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China; Longmen Animal Disease Prevention and Control Center, 516800, Huizhou, Guangdong, China.
Dev Biol. 2023 Sep;501:104-110. doi: 10.1016/j.ydbio.2023.04.004. Epub 2023 May 12.
A healthy mammary gland is a necessity for milk production of dairy goats. The role of chi-miR-3880 in goat lactation is illustrated in our previous study. Among the differentially expressed genes regulated by chi-miR-3880, one seventh were interferon stimulated genes, including MX1, MX2, IFIT3, IFI44L, and DDX58. As the inflammatory cytokine interferon gamma (IFNγ) has been identified as a potential marker of caseous lymphadenitis in lactating sheep, the interaction between IFNγ and immune-related microRNAs was explored in this study. Chi-miR-3880 was found to be one of the microRNAs downregulated by IFNγ in goat mammary epithelial cells (GMECs). The study illustrated that IFNγ/chi-miR-3880/DDX58 axis modulates GMEC proliferation and lipid formation through PI3K/AKT/mTOR pathway, and regulates apoptosis through Caspase-3 and Bcl-2/Bax pathways. The role of the axis in mammary involution was reflected by the expression of p53 and NF-κB. In conclusion, IFNγ/chi-miR-3880/DDX58 axis plays an important part in lactation.
健康的乳腺是奶山羊产奶的必要条件。我们之前的研究阐述了chi-miR-3880在山羊泌乳中的作用。在受chi-miR-3880调控的差异表达基因中，七分之一是干扰素刺激基因，包括MX1、MX2、IFIT3、IFI44L和DDX58。由于炎性细胞因子γ干扰素（IFNγ）已被确定为泌乳绵羊干酪性淋巴结炎的潜在标志物，本研究探讨了IFNγ与免疫相关微小RNA之间的相互作用。发现chi-miR-3880是山羊乳腺上皮细胞（GMECs）中受IFNγ下调的微小RNA之一。该研究表明，IFNγ/chi-miR-3880/DDX58轴通过PI3K/AKT/mTOR途径调节GMEC增殖和脂质形成，并通过Caspase-3和Bcl-2/Bax途径调节细胞凋亡。p53和NF-κB的表达反映了该轴在乳腺退化中的作用。总之，IFNγ/chi-miR-3880/DDX58轴在泌乳中起重要作用。
