Birkeland Nils-Kåre, Bunk Boyke, Spröer Cathrin, Klenk Hans-Peter, Schönheit Peter
Department of Biological Sciences, University of Bergen, N-5020 Bergen, Norway.
Department Bioinformatics and Databases, Leibniz Institute DSMZ, D-38124 Braunschweig, Germany.
Biology (Basel). 2021 Apr 29;10(5):387. doi: 10.3390/biology10050387.
A novel hyperthermophilic archaeon, termed strain T7324, was isolated from a mixed sulfate-reducing consortium recovered from hot water produced from a deep North Sea oil reservoir. The isolate is a strict anaerobic chemo-organotroph able to utilize yeast extract or starch as a carbon source. The genes for a number of sugar degradation enzymes and glutamate dehydrogenase previously attributed to the sulfate reducing strain of the consortium ( strain 7324) were identified in the nearly completed genome sequence. Sequence analysis of the 16S rRNA gene placed the strain in the genus, but with an average nucleotide identity that is less than 90% to its closest relatives. Phylogenomic treeing reconstructions placed the strain on a distinct lineage clearly separated from other spp. The results indicate that the strain T7324 represents a novel species, for which the name sp. nov. is proposed. The type strain is T7324 (=DSM 27149 = KCTC 15808).
从北海深层油藏产出的热水中分离得到的一个混合硫酸盐还原菌群中，分离出了一种新型嗜热古菌，命名为菌株T7324。该分离株是一种严格厌氧的化能有机营养菌，能够利用酵母提取物或淀粉作为碳源。在几乎完整的基因组序列中鉴定出了一些先前归因于该菌群硫酸盐还原菌株（菌株7324）的糖降解酶和谷氨酸脱氢酶基因。16S rRNA基因的序列分析将该菌株归入该属，但与其最亲近的亲属的平均核苷酸同一性低于90%。系统发育基因组树重建将该菌株置于一个与其他物种明显分离的独特谱系上。结果表明，菌株T7324代表一个新物种，为此提出新物种名称。模式菌株为T7324（=DSM 27149 = KCTC 15808）。
