构建猪流行性腹泻病毒重组乳酸乳球菌疫苗及其在小鼠中的免疫应答。

The construction of recombinant Lactobacillus casei vaccine of PEDV and its immune responses in mice.

机构信息

Swine Research Institute of New Hope Group, Tai'an, China.

Shandong Swine Herd Health Big Data and Intelligent Monitoring Engineering Laboratory, Tai'an, China.

出版信息

BMC Vet Res. 2021 May 4;17(1):184. doi: 10.1186/s12917-021-02885-y.

BACKGROUND

Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant L. casei-OMP16-PEDVS strain expressing PEDVS protein of PEDV and OMP16 protein of Brucella abortus strain. To know the immunogenicity of the recombinant L. casei-OMP16-PEDVS candidate vaccine, it was compared with BL21-OMP16-PEDVS-F, BL21-OMP16-PEDVS, and BL21-PEDVS recombinant protein.

RESULTS

The results showed that we could detect higher levels of IgG, neutralizing antibody, IL-4, IL-10, and INF-γ in serum and IgA in feces of L. casei-OMP16-PEDVS immunized mice, which indicated that L. casei-OMP16-PEDVS candidate vaccine could induce higher levels of humoral immunity, cellular immunity, and mucosal immunity.

CONCLUSION

Therefore, L. casei-OMP16-PEDVS is a promising candidate vaccine for prophylaxis of PEDV infection.

背景

猪流行性腹泻（PED）是一种由猪流行性腹泻病毒（PEDV）引起的传染性肠道疾病，其特征为呕吐、腹泻、食欲不振和脱水，这在世界范围内造成了巨大的经济损失。目前，疫苗免疫仍然是控制 PED 传播的最有效方法。在本研究中，我们构建了一种新型重组乳杆菌-OMP16-PEDVS 菌株，表达 PEDV 蛋白和布鲁氏菌 abortus 菌株的 OMP16 蛋白。为了了解重组乳杆菌-OMP16-PEDVS 候选疫苗的免疫原性，我们将其与 BL21-OMP16-PEDVS-F、BL21-OMP16-PEDVS 和 BL21-PEDVS 重组蛋白进行了比较。