Dai Jiewen, Fu Yuanfei, Chen Demin, Sun Zhaoyao
Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Department of Prosthodontics, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Mater Sci Eng C Mater Biol Appl. 2021 May;124:112052. doi: 10.1016/j.msec.2021.112052. Epub 2021 Mar 25.
Reconstruction of bone defects is still a challenge. In this study, we developed and systematically evaluated a novel injectable strontium-containing hydroxyapatite (Sr-HA) bone cement in which Sr-HA powder included 5% Sr and was mixed with a setting liquid that included 5% potassium citrate. This Sr-HA cement was mainly composed of HA and α-tricalcium phosphate (TCP) and exhibited favorable injectability (100%), setting times (the initial setting time was 240 s and the final setting time was 420 s), compressive strength (73.4 MPa), maximal load and maximum bending stress, and excellent radiopacity. In addition, the Sr-HA cement also had excellent biocompatibility that exhibited low cytotoxicity for cell proliferation and no obvious disturbing effect on the osteogenic differentiation of periodontal ligament stem cells (DLSCs) and dental pulp stem cells (DPSCs). However, the Sr-HA cement could slightly promote the osteogenic differentiation of MC3T3 cells, which also implied that it would promote osseointegration between the cement and surrounding bone but would not obviously disturb the biological behavior of DLSCs and DPSCs. An in vivo study further confirmed that Sr-HA cement exhibited favorable osseointegration with the maxilla and tibia. All these findings implied that the novel Sr-HA cement was a suitable bone substitution for bone defects.
骨缺损的修复仍然是一项挑战。在本研究中，我们研发并系统评估了一种新型可注射含锶羟基磷灰石（Sr-HA）骨水泥，其中Sr-HA粉末含有5%的锶，并与含有5%柠檬酸钾的固化液混合。这种Sr-HA骨水泥主要由羟基磷灰石（HA）和α-磷酸三钙（TCP）组成，具有良好的可注射性（100%）、凝固时间（初始凝固时间为240秒，最终凝固时间为420秒）、抗压强度（73.4兆帕）、最大载荷和最大弯曲应力，以及出色的射线不透性。此外，Sr-HA骨水泥还具有优异的生物相容性，对细胞增殖表现出低细胞毒性，对牙周膜干细胞（DLSCs）和成牙本质细胞（DPSCs）的成骨分化没有明显干扰作用。然而，Sr-HA骨水泥能轻微促进MC3T3细胞的成骨分化，这也意味着它会促进骨水泥与周围骨之间的骨整合，但不会明显干扰DLSCs和DPSCs的生物学行为。一项体内研究进一步证实，Sr-HA骨水泥与上颌骨和胫骨表现出良好的骨整合。所有这些发现表明，新型Sr-HA骨水泥是一种适用于骨缺损的骨替代物。
