Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.
Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, FI-00014 Helsinki, Finland.
Mater Sci Eng C Mater Biol Appl. 2021 May;124:112079. doi: 10.1016/j.msec.2021.112079. Epub 2021 Mar 27.
Electrospun organic/inorganic hybrid scaffolds have been appealing in tissue regeneration owing to the integrated physicochemical and biological performances. However, the conventional electrospun scaffolds with non-woven structures usually failed to enable deep cell infiltration due to the densely stacked layers among the fibers. Herein, through self-assembly-driven electrospinning, a polyhydroxybutyrate/poly(ε-caprolactone)/58S sol-gel bioactive glass (PHB/PCL/58S) hybrid scaffold with honeycomb-like structures was prepared by manipulating the solution composition and concentration during a one-step electrospinning process. The mechanisms enabling the formation of self-assembled honeycomb-like structures were investigated through comparative studies using Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) between PHB/PCL/58S and PHB/PCL/sol-gel silica systems. The obtained honeycomb-like structure was built up from nanofibers with an average diameter of 370 nm and showed a bimodal distribution of pores: large polygonal pores up to hundreds of micrometers within the honeycomb-cells and irregular pores among the nanofibers ranging around few micrometers. The cell-materials interactions were further studied by culturing MG-63 osteoblast-like cells for 7 days. Cell viability, cell morphology and cell infiltration were comparatively investigated as well. While cells merely proliferated on the surface of non-woven structures, MG-63 cells showed extensive proliferation and deep infiltration up to 100-200 μm into the honeycomb-like structure. Moreover, the cellular spatial organization was readily regulated by the honeycomb-like pattern as well. Overall, the newly obtained hybrid scaffold may integrate the enhanced osteogenicity originating from the bioactive components, and the improved cell-material interactions brought by the honeycomb-like structure, making the new scaffold a promising candidate for tissue regeneration.
静电纺丝有机/无机杂化支架由于其综合的物理化学和生物学性能，在组织再生中受到关注。然而，由于纤维之间的密集堆叠层，传统的具有无纺结构的静电纺丝支架通常无法实现深层细胞渗透。在此，通过自组装驱动的静电纺丝，通过在一步静电纺丝过程中操纵溶液组成和浓度，制备了具有蜂窝状结构的聚羟基丁酸酯/聚（ε-己内酯）/58S 溶胶-凝胶生物活性玻璃（PHB/PCL/58S）杂化支架。通过傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）和热重分析（TGA）对 PHB/PCL/58S 和 PHB/PCL/溶胶-凝胶二氧化硅系统进行对比研究，探讨了形成自组装蜂窝状结构的机制。所得的蜂窝状结构由平均直径为 370nm 的纳米纤维组成，并表现出大孔和小孔的双峰分布：蜂窝细胞内的大多边形孔可达数百微米，纳米纤维之间的不规则孔约为几微米。通过培养 MG-63 成骨样细胞 7 天进一步研究细胞-材料相互作用。比较研究了细胞活力、细胞形态和细胞渗透。当细胞仅在无纺结构表面增殖时，MG-63 细胞表现出广泛的增殖和深层渗透，可达 100-200μm 进入蜂窝状结构。此外，蜂窝状图案还可以容易地调节细胞的空间组织。总体而言，新获得的杂化支架可能整合了生物活性成分增强的成骨活性，以及蜂窝状结构带来的改善的细胞-材料相互作用，使新支架成为组织再生的有前途的候选物。
