Department of Materials Engineering, Faculty of Engineering, Ferdowsi University of Mashhad (FUM), Azadi Sq., Mashhad 917794-8564, Iran.
Department of Materials Engineering, Faculty of Engineering, Ferdowsi University of Mashhad (FUM), Azadi Sq., Mashhad 917794-8564, Iran.
Mater Sci Eng C Mater Biol Appl. 2021 May;124:112082. doi: 10.1016/j.msec.2021.112082. Epub 2021 Mar 29.
Surface treatment of biomaterials could enable reliable and quick cellular responses and accelerate the healing of the host tissue. Here, a series of calcium phosphates (CaPs) were surface treated by hydrogen peroxide (HO) and the treatment effects were physicochemically and biologically evaluated. For this aim, as-synthesized CaPs doped with strontium (Sr), iron (Fe), silicon (Si), and titanium (Ti) ions were sonicated in HO media. The results showed that the specific surface area and zeta potential values of the surface-treated CaPs were increased by ~50% and 25%, respectively. Moreover, the particle size and the band-gap (Eg) values of the surface-treated CaPs were decreased by ~25% and ~2-10%, respectively. The concentration of oxygen vacancies was increased in the surface-treated samples, which was confirmed by the result of ultraviolet (UV), photoluminescence (PL), Commission Internationale de l'éclairage (CIE 1931), and X-ray photoelectron spectroscopy (XPS) analyses. In vitro cellular assessments of surface-treated CaPs exhibited an improvement in cytocompatibility, reactive oxygen species generation (ROS) capacity, bone nodule formation, and the migration of cells up to ~8%, 20%, 35%, and 13%, respectively. Based on the obtained data, it can be stated that improved physicochemical properties of HO-treated CaPs could increase the ROS generation and subsequently enhance the biological activities. In summary, the results demonstrate the notable effect of the HO surface treatment method on improving surface properties and biological performance of CaPs.
生物材料的表面处理可以实现可靠、快速的细胞响应，并加速宿主组织的愈合。在这里，通过过氧化氢（HO）对一系列钙磷（CaP）进行了表面处理，并对处理效果进行了物理化学和生物学评估。为此，在 HO 介质中对合成的掺锶（Sr）、铁（Fe）、硅（Si）和钛（Ti）离子的 CaP 进行了超声处理。结果表明，表面处理 CaP 的比表面积和zeta 电位值分别增加了约 50%和 25%。此外，表面处理 CaP 的粒径和带隙（Eg）值分别降低了约 25%和 2-10%。表面处理样品中的氧空位浓度增加，这通过紫外线（UV）、光致发光（PL）、国际照明委员会（CIE 1931）和 X 射线光电子能谱（XPS）分析得到证实。表面处理 CaP 的体外细胞评估显示，细胞相容性、活性氧（ROS）生成能力、骨结节形成和细胞迁移分别提高了约 8%、20%、35%和 13%。基于获得的数据，可以说 HO 处理 CaP 的物理化学性质的改善可以增加 ROS 的生成，从而增强其生物活性。总之，结果表明 HO 表面处理方法对改善 CaP 的表面性能和生物性能具有显著效果。
