Akintunde J K, Farai T I, Arogundade M R, Adeleke J T
Applied Biochemistry and Molecular Toxicology Research Group, Department of Biochemistry, College of Biosciences, Federal University of Agriculture, Abeokuta, Nigeria.
Toxicology and Safety Unit, Department of Environmental Health Sciences, Faculty of Public Health, College of Medicine, University of Ibadan, Nigeria.
Biochem Biophys Rep. 2021 Apr 17;26:100999. doi: 10.1016/j.bbrep.2021.100999. eCollection 2021 Jul.
Zinc oxide nanoparticles (ZnONPs) from plant origin were postulated to regulate complex hormonal control through the hypothalamus- pituitary-testicular axis and somatic cells due to their unique small size and effective drug delivery to target tissues. This study therefore investigates the biogenic synthesis of zinc oxide nanoparticles (ZnO NPs) from leaves on key endocrine hormones (LH, FSH and testosterone), MDA level, antioxidant enzymes (SOD and CAT), acetylcholineesterase (AChE) activity and reactive nitrogen species (NO) level in rotenone induced male rat. The animals were divided into six groups (n = 8). Group I was orally given olive oil as vehicle; Group II received 60 mg/kg of rotenone (RTNE) only; Group III (RTNE + ZnONPs) received 60 mg/kg RTNE + 10 mg/kg ZnONPs; Group IV (RTNE + ZnCAP) received 60 mg/kg RTNE + 50 mg/kg zinc capsule; Group V (ZnONPs only) received 10 mg/kg ZnONPs only. Group VI received 50 mg/kg ZnCAP only. The experiment lasted 10 days. TEM and XRD images revealed ZnO NPs. Moreover, the presence of organic molecules in bio-reduction reactions from the FTIR spectrum showed the stabilization of the nanoparticles. Also, animals induced with rotenone exhibited impairment in the leydig cells by depleting LH, FSH, and testosterone levels with reduced AChE activity and significant (p < 0.05) alteration in cerebral enzymatic antioxidants. There was also brain increase in NO production: marker of pro-inflammation. Nanotherapeutically, ZnONPs regulated hypothalamus-pituitary-testicular axis via modulation of cerebral NO, FSH, LH, testosterone and AChE activity with induction of anti-oxidative enzymes.
源自植物的氧化锌纳米颗粒（ZnONPs）因其独特的小尺寸和对靶组织有效的药物递送，被假定可通过下丘脑 - 垂体 - 睾丸轴和体细胞调节复杂的激素控制。因此，本研究调查了从树叶生物合成的氧化锌纳米颗粒（ZnO NPs）对鱼藤酮诱导的雄性大鼠关键内分泌激素（LH、FSH和睾酮）、丙二醛（MDA）水平、抗氧化酶（SOD和CAT）、乙酰胆碱酯酶（AChE）活性和活性氮物质（NO）水平的影响。将动物分为六组（n = 8）。第一组口服橄榄油作为赋形剂；第二组仅接受60 mg/kg的鱼藤酮（RTNE）；第三组（RTNE + ZnONPs）接受60 mg/kg RTNE + 10 mg/kg ZnONPs；第四组（RTNE + ZnCAP）接受60 mg/kg RTNE + 50 mg/kg锌胶囊；第五组（仅ZnONPs）仅接受10 mg/kg ZnONPs。第六组仅接受50 mg/kg ZnCAP。实验持续10天。透射电子显微镜（TEM）和X射线衍射（XRD）图像显示了ZnO NPs。此外，傅里叶变换红外光谱（FTIR）谱中生物还原反应中有机分子的存在表明了纳米颗粒的稳定性。同样，用鱼藤酮诱导的动物表现出睾丸间质细胞受损，LH、FSH和睾酮水平降低，AChE活性降低，脑内酶促抗氧化剂有显著（p < 0.05）改变。脑内NO生成也增加：这是促炎的标志物。在纳米治疗方面，ZnONPs通过调节脑内NO、FSH、LH、睾酮和AChE活性以及诱导抗氧化酶来调节下丘脑 - 垂体 - 睾丸轴。
