Khatri Dharmendra K, Juvekar Archana R
Pharmacology Research Laboratory-I, Dept. of Pharm. Sci. & Tech, Institute of Chemical Technology, (University under Section 3 of UGC Act- 1956, Elite Status & Centre of Excellence -Govt. of Maharashtra, TEQIP Phase II Funded), India.
Pharmacology Research Laboratory-I, Dept. of Pharm. Sci. & Tech, Institute of Chemical Technology, (University under Section 3 of UGC Act- 1956, Elite Status & Centre of Excellence -Govt. of Maharashtra, TEQIP Phase II Funded), India.
Pharmacol Biochem Behav. 2016 Nov-Dec;150-151:39-47. doi: 10.1016/j.pbb.2016.09.002. Epub 2016 Sep 9.
Curcumin, a natural polyphenolic compound extracted from rhizomes of Curcuma longa (turmeric), a plant in the ginger family (Zingiberaceae) has been used worldwide and extensively in Southeast Asia. Curcumin exhibited numerous biological and pharmacological activities including potent antioxidant, cardiovascular disease, anticancer, anti-inflammatory effects and neurodegenerative disorders in cell cultures and animal models. Hence, the present study was designed in order to explore the possible neuroprotective role of curcumin against rotenone induced cognitive impairment, oxidative and mitochondrial dysfunction in mice. Chronic administration of rotenone (1mg/kg i.p.) for a period of three weeks significantly impaired cognitive function (actophotometer, rotarod and open field test), oxidative defense (increased lipid peroxidation, nitrite concentration and decreased activity of superoxide dismutase, catalase and reduced glutathione level) and mitochondrial complex (II and III) enzymes activities as compared to normal control group. Three weeks of curcumin (50, 100 and 200mg/kg, p.o.) treatment significantly improved behavioral alterations, oxidative damage and mitochondrial enzyme complex activities as compared to negative control (rotenone treated) group. Curcumin treated mice also mitigated enhanced acetylcholine esterase enzyme level as compared to negative control group. We found that curcumin restored motor deficits and enhanced the activities of antioxidant enzymes suggesting its antioxidant potential in vivo. The findings of the present study conclude neuroprotective role of curcumin against rotenone induced Parkinson's in mice and offer strong justification for the therapeutic prospective of this compound in the management of PD.
姜黄素是从姜科植物姜黄的根茎中提取的一种天然多酚化合物，在全球范围内广泛使用，在东南亚地区使用尤为广泛。在细胞培养和动物模型中，姜黄素表现出多种生物学和药理活性，包括强大的抗氧化、心血管疾病、抗癌、抗炎作用以及对神经退行性疾病的作用。因此，本研究旨在探讨姜黄素对鱼藤酮诱导的小鼠认知障碍、氧化和线粒体功能障碍可能具有的神经保护作用。与正常对照组相比，连续三周腹腔注射鱼藤酮（1mg/kg）显著损害了认知功能（光电计、转棒试验和旷场试验）、氧化防御能力（脂质过氧化增加、亚硝酸盐浓度升高，超氧化物歧化酶、过氧化氢酶活性降低以及还原型谷胱甘肽水平降低）和线粒体复合物（II和III）酶活性。与阴性对照组（鱼藤酮处理组）相比，连续三周口服姜黄素（50、100和200mg/kg）治疗显著改善了行为改变、氧化损伤和线粒体酶复合物活性。与阴性对照组相比，姜黄素处理的小鼠还减轻了乙酰胆碱酯酶水平的升高。我们发现姜黄素恢复了运动功能缺陷并增强了抗氧化酶的活性，表明其在体内具有抗氧化潜力。本研究结果表明姜黄素对鱼藤酮诱导的小鼠帕金森病具有神经保护作用，并为该化合物在帕金森病治疗中的前景提供了有力的依据。
