Cellular and Molecular Research Center, Gerash University of Medical Sciences, Gerash, Iran.
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Reprod Sci. 2021 Dec;28(12):3491-3497. doi: 10.1007/s43032-021-00598-z. Epub 2021 May 4.
Endometriosis is a chronic inflammatory disease that has been reported to be associated with immune system dysfunction. On the other hand, the effect of Vitamin D as an immune modulator and its relation with several autoimmune and inflammatory diseases has been previously investigated. Moreover, several studies have reported the polymorphisms of VDR and VDBP genes can change the functions of these molecules. Therefore, these polymorphisms may be influential on endometriosis pathogenesis. In this study, we aimed at evaluating the association between VDR gene (FokI (F/f), BsmI (B/b), ApaI (A/a), TaqI (T/t)), and VDBP gene (GC1S, GC1F, and GC*2) polymorphisms with endometriosis in Iranian women population. This case-control study was performed on 120 women with endometriosis and 110 healthy women. ARMS-PCR and PCR-RFLP methods were used to inspect polymorphisms in VDR and VDBP genes, respectively. Based on the results, there was no statistically significant difference between the cases with endometriosis and control subjects in terms of genotypes and allele frequencies of VDR and VDBP gene polymorphisms. These data suggest that VDR and VDBP gene polymorphisms may have no role in endometriosis susceptibility in Iranian women.
子宫内膜异位症是一种慢性炎症性疾病，据报道与免疫系统功能障碍有关。另一方面，维生素 D 作为免疫调节剂的作用及其与几种自身免疫和炎症性疾病的关系已被先前研究。此外，一些研究报告称 VDR 和 VDBP 基因的多态性可以改变这些分子的功能。因此，这些多态性可能对子宫内膜异位症的发病机制有影响。在这项研究中，我们旨在评估 VDR 基因（FokI（F/f）、BsmI（B/b）、ApaI（A/a）、TaqI（T/t））和 VDBP 基因（GC1S、GC1F 和 GC*2）多态性与伊朗女性子宫内膜异位症之间的关联。这项病例对照研究共纳入了 120 名子宫内膜异位症患者和 110 名健康女性。使用 ARMS-PCR 和 PCR-RFLP 方法分别检测 VDR 和 VDBP 基因的多态性。基于研究结果，在 VDR 和 VDBP 基因多态性的基因型和等位基因频率方面，子宫内膜异位症患者与对照组之间无统计学差异。这些数据表明，VDR 和 VDBP 基因多态性可能与伊朗女性子宫内膜异位症的易感性无关。
