Department of Immunology and Allergology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
Lab of Immunology, Department of Immunology and Allergology, Lithuanian University of Health Sciences, Kaunas, Lithuania.
BMC Pulm Med. 2023 Jul 5;23(1):245. doi: 10.1186/s12890-023-02531-3.
The effects of vitamin D are exerted by interaction with the vitamin D receptor (VDR) and vitamin D binding protein (VDBP). Polymorphisms in VDR or VDBP genes may affect vitamin D levels, influencing the pathogenesis of asthma and atopy. The aim of this study was to investigate the possible association of VDR and VDBP gene single-nucleotide polymorphisms (SNP), 25-hydroxyvitamin D (25(OH)D), blood eosinophils and total IgE level in subjects with asthma in comparison with healthy individuals.
This case-control study enrolled 63 subjects with asthma (45 allergic and 18 non-allergic) and 32 healthy subjects were involved in the study. Sensitization of subjects to inhaled allergens was determined by a skin prick test, lung function was evaluated by spirometry. Blood eosinophil count was determined by standard methods. Serum 25(OH)D and total IgE levels were evaluated by ELISA. Polymorphisms in the VDR and VDBP genes on the 12q13.11 and 4q13.3 chromosomal region were analyzed using TaqMan SNP Genotyping Assay probes.
In asthma patients with vitamin D deficiency (< 20 ng/ml) the allele G of rs11168293 of VDR was more common than in those having insufficiency (20-30 ng/ml) of vitamin D (63% and 31%, p < 0.05). Moreover, asthmatic subject with rs11168293 G allele has significant higher blood eosinophil count compared to asthmatic without the rs11168293 G allele (8.5 ± 12.3% vs. 5.1 ± 1.5%, p < 0.05). Significantly higher IgE level was found in subjects with allergic asthma with the allele A of rs7041 on VDBP gene than in those without this allele (540 ± 110 and 240 ± 80 IU/ml, p < 0.05).
The association of polymorphisms in VDBP and VDR gene, the rs11168293 G allele and the rs7041 A allele, with 25(OH)D, blood eosinophil and total IgE level in asthma, let us suggest that vitamin D, VDR and VDBP gene polymorphisms are important in pathogenesis of asthma despite its form in relation to atopy.
维生素 D 的作用是通过与维生素 D 受体 (VDR) 和维生素 D 结合蛋白 (VDBP) 的相互作用发挥的。VDR 或 VDBP 基因的多态性可能会影响维生素 D 水平，从而影响哮喘和特应性的发病机制。本研究旨在探讨维生素 D 受体 (VDR) 和维生素 D 结合蛋白 (VDBP) 基因单核苷酸多态性 (SNP)、25-羟维生素 D(25(OH)D)、血嗜酸性粒细胞和总 IgE 水平与哮喘患者之间的可能关联，并与健康个体进行比较。
本病例对照研究纳入了 63 例哮喘患者(45 例过敏性和 18 例非过敏性)和 32 例健康受试者。采用皮肤点刺试验确定受试者对吸入性过敏原的致敏情况，通过肺功能测定评估肺功能。采用标准方法测定血嗜酸性粒细胞计数。采用 ELISA 法测定血清 25(OH)D 和总 IgE 水平。采用 TaqMan SNP 基因分型检测探针分析 12q13.11 和 4q13.3 染色体区域的 VDR 和 VDBP 基因多态性。
在维生素 D 缺乏(<20ng/ml)的哮喘患者中，VDR 基因 rs11168293 的等位基因 G 比维生素 D 不足(20-30ng/ml)的患者更为常见(63%和 31%，p<0.05)。此外，携带 rs11168293 G 等位基因的哮喘患者的血嗜酸性粒细胞计数明显高于不携带 rs11168293 G 等位基因的哮喘患者(8.5±12.3%比 5.1±1.5%，p<0.05)。在 VDBP 基因 rs7041 等位基因 A 的过敏性哮喘患者中，IgE 水平显著高于无该等位基因的患者(540±110 和 240±80IU/ml，p<0.05)。
VDBP 和 VDR 基因多态性、rs11168293 G 等位基因和 rs7041 A 等位基因与 25(OH)D、血嗜酸性粒细胞和总 IgE 水平在哮喘中的关联，提示尽管与特应性有关，但维生素 D、VDR 和 VDBP 基因多态性在哮喘发病机制中很重要。
