Lipides: systèmes analytiques et biologiques, Université Paris-Saclay, 92296 Châtenay-Malabry, France.
Analyst. 2021 May 4;146(9):2965-2973. doi: 10.1039/d1an00231g.
From the basal layer until the stratum corneum, lipid and protein biomarkers associated with morphological changes denote keratinocyte differentiation and characterize each epidermis layer. Herein, we followed keratinocyte differentiation in the early stages using HaCaT cells over a period of two weeks by two complementary analytical techniques: Raman microspectroscopy and high-performance liquid chromatography coupled with high resolution mass spectrometry. A high concentration of calcium in the medium induced HaCaT cell differentiation in vitro. The results from both techniques underlined the keratinocyte passage from the granular layer (day 9) to the stratum corneum layer (day 13). After 13 days of differentiation, we observed a strong increase in the lipid content, decrease in proteins, decrease in DNA, and a decrease in glucosylceramides/ceramides and sphingomyelins/ceramides ratios.
从基底层到角质层，与形态变化相关的脂质和蛋白质生物标志物表明角质形成细胞分化，并表征每个表皮层。在此，我们使用 HaCaT 细胞，通过两种互补的分析技术（拉曼微光谱和高效液相色谱与高分辨率质谱联用），在两周的时间内跟踪角质形成细胞在早期的分化。培养基中高浓度的钙诱导 HaCaT 细胞体外分化。这两种技术的结果都强调了角质形成细胞从颗粒层（第 9 天）到角质层（第 13 天）的转变。分化 13 天后，我们观察到脂质含量增加，蛋白质减少，DNA 减少，以及葡萄糖神经酰胺/神经酰胺和神经鞘氨醇/神经酰胺的比例降低。
