Faculty of Dental Surgery, Department of Oral Surgery, University of Strasbourg, Strasbourg, France.
Department of Dentistry and oral Health, Division of Oral Surgery and Orthodontics, Medical University of Graz, Graz, Austria.
Clin Exp Dent Res. 2021 Oct;7(5):711-718. doi: 10.1002/cre2.414. Epub 2021 May 5.
The effectiveness of GBR procedures for the reconstruction of periodontal defects has been well documented. The objective of this investigation was to evaluate the degradation kinetics and biocompatibility of two resorbable collagen membranes in conjunction with a bovine xenograft material.
Lower premolars and first molars were extracted from 18 male Yucatan minipigs. After 4 months of healing, standardized semi-saddle defects were created (12 mm × 8 mm × 8 mm [l˙̇ × W˙ × d]), with 10 mm between adjacent defects. The defects were filled with a bovine xenograft and covered with a either the bilayer collagen membrane (control) or the porcine pericardium-derived collagen membrane (test). Histological analysis was performed after 4, 8, and 12 weeks of healing and the amount of residual membrane evaluated. Non-inferiority was calculated using the Brunner-Langer mixed regression model.
Histological analysis indicated the presence of residual membrane in both groups at all time points, with significant degradation noted in both groups at 12 weeks compared to 4 weeks (p = .017). No significant difference in ranked residual membrane scores between the control and test membranes was detected at any time point.
The pericardium-derived membrane was shown to be statistically non-inferior to the control membrane with respect to resorption kinetics and barrier function when utilized for guided bone regeneration in semi-saddle defects in minipigs. Further evaluation is necessary in the clinical setting.
GBR 程序在牙周缺损重建中的有效性已得到充分证实。本研究旨在评估两种可吸收胶原膜与牛异种移植物联合使用时的降解动力学和生物相容性。
从 18 只雄性尤卡坦小型猪中提取下颌前磨牙和第一磨牙。愈合 4 个月后，创建标准化的半鞍形缺损（12mm×8mm×8mm[l˙×W˙×d]），相邻缺损之间间隔 10mm。用牛异种移植物填充缺损，并用双层胶原膜（对照）或猪心包衍生胶原膜（试验）覆盖。在愈合后 4、8 和 12 周进行组织学分析，并评估残留膜的量。使用 Brunner-Langer 混合回归模型计算非劣效性。
组织学分析表明，两组在所有时间点均存在残留膜，与 4 周相比，两组在 12 周时均出现明显降解（p=0.017）。在任何时间点，对照膜和试验膜的残留膜评分均无显著差异。
在心包衍生膜用于小型猪半鞍形缺损引导性骨再生时，其在吸收动力学和屏障功能方面被证明在统计学上不劣于对照膜。在临床环境中需要进一步评估。
