Sehgal Vishal, Ruangsawasdi Nisarat, Kiattavorncharoen Sirichai, Bencharit Sompop, Thanasrisuebwong Prakan
Master of Science Program in Implant Dentistry, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand.
Materials (Basel). 2023 Feb 16;16(4):1657. doi: 10.3390/ma16041657.
Different collagen barrier membranes come in various sources and crosslinking that may affect barrier function and tissue integration. This study investigated barrier function and tissue integration of the three different collagen membranes (Jason: porcine pericardium, GENOSS: bovine tendon, and BioMend Extend: cross-linked bovine tendon) with human gingival fibroblasts. The barrier function and tissue integration properties were determined under confocal microscopy. Morphological characteristics were observed using scanning electron microscopy. Our results showed that all collagen membranes allowed a small number of cells to migrate, and the difference in barrier function ability was not significant. The cross-linked characteristics did not improve barrier ability. The native collagen membrane surfaces allowed evenly scattered proliferation of HGF, while the cross-linked collagen membrane induced patchy proliferation. Statistically significant differences in cell proliferation were found between Jason and BioMend Extend membranes ( = 0.04). Scanning electron microscope showed a compact membrane surface at the top, while the bottom surfaces displayed interwoven collagen fibers, which were denser in the crosslinked collagen membranes. Within the limitations of this study, collagen membranes of different origins and physical properties can adequately prevent the invasion of unwanted cells. Native collagen membranes may provide a better surface for gingival cell attachment and proliferation.
不同的胶原屏障膜有多种来源和交联方式，这可能会影响屏障功能和组织整合。本研究调查了三种不同的胶原膜（Jason：猪心包膜、GENOSS：牛肌腱、BioMend Extend：交联牛肌腱）与人牙龈成纤维细胞的屏障功能和组织整合情况。在共聚焦显微镜下测定屏障功能和组织整合特性。使用扫描电子显微镜观察形态特征。我们的结果表明，所有胶原膜都允许少量细胞迁移，屏障功能能力的差异不显著。交联特性并未提高屏障能力。天然胶原膜表面允许人牙龈成纤维细胞均匀分散增殖，而交联胶原膜诱导斑块状增殖。在Jason膜和BioMend Extend膜之间发现细胞增殖存在统计学显著差异（P = 0.04）。扫描电子显微镜显示顶部膜表面致密，而底部表面呈现交织的胶原纤维，在交联胶原膜中更密集。在本研究的局限性内，不同来源和物理性质的胶原膜能够充分防止不需要的细胞侵入。天然胶原膜可能为牙龈细胞附着和增殖提供更好的表面。
