Talebi Ardakani Mohammad Reza, Hajizadeh Farhad, Yadegari Zahra
Department of Periodontology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ann Maxillofac Surg. 2018 Jul-Dec;8(2):218-223. doi: 10.4103/ams.ams_150_17.
Human gingival fibroblasts cultured on collagen membrane as an alternative treatment method used in tissue regeneration can lead to improved results in root coverage. The aim of this study was to evaluate the human gingival fibroblast proliferation and adhesion cultured on three types of collagen membranes.
In this study, first-line human gingival fibroblast cells (HGF1-RT1) prepared and cultured on three membranes, including porcine pericardium (PP) (Jason, Botiss dental), human pericardium (HP) (Regen, Faravardeh Baft Iranian), and glutaraldehyde cross-linked (GC) (BioMend Extend, Zimmer Dental). Cell survival was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) after 24, 48, and 72 h and 7 days. Furthermore, morphology and adhesion of cells on the membrane were evaluated after 1 and 7 days by electron microscopy (scanning electron microscopy [SEM]). Statistical analysis was performed using two-way ANOVA with a significance level of 0.05.
Based on the results of MTT, cell survival on HP and PP membranes after 7 days significantly increased ( < 0.001), but for the GC membrane, it was reduced after 7 days ( = 0.031). Cell survival on HP and PP membranes did not differ ( = 1) and was more than GC ( < 0.001). SEM images showed that the adhesion of cells was better on HP and PP membranes than GC.
The results of this study showed that natural collagen membranes (HP and PP) similarly support proliferation and adhesion of gingival fibroblasts. Survival and adhesion of gingival fibroblasts on cross-linked collagen membrane was less than two other membranes.
在胶原膜上培养人牙龈成纤维细胞作为组织再生的一种替代治疗方法，可在牙根覆盖方面取得更好的效果。本研究旨在评估在三种类型胶原膜上培养的人牙龈成纤维细胞的增殖和黏附情况。
在本研究中，将原代人牙龈成纤维细胞（HGF1-RT1）制备并培养在三种膜上，包括猪心包膜（PP）（Jason，Botiss牙科）、人心包膜（HP）（Regen，Faravardeh Baft伊朗公司）和戊二醛交联（GC）（BioMend Extend，Zimmer牙科）。在24、48、72小时及7天后，使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑（MTT）评估细胞存活率。此外，在1天和7天后通过电子显微镜（扫描电子显微镜[SEM]）评估细胞在膜上的形态和黏附情况。使用双向方差分析进行统计分析，显著性水平为0.05。
基于MTT结果，7天后HP和PP膜上的细胞存活率显著增加（P<0.001），但对于GC膜，7天后细胞存活率降低（P = 0.031）。HP和PP膜上的细胞存活率无差异（P = 1），且高于GC膜（P<0.001）。SEM图像显示，细胞在HP和PP膜上的黏附比在GC膜上更好。
本研究结果表明，天然胶原膜（HP和PP）同样支持牙龈成纤维细胞的增殖和黏附。交联胶原膜上牙龈成纤维细胞的存活和黏附低于其他两种膜。
