Department of Biology, School of Mathematics and Natural Sciences, Mongolian National University of Education, Ulaanbaatar, Mongolia.
Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Arch Virol. 2021 Jul;166(7):2051-2054. doi: 10.1007/s00705-021-05081-3. Epub 2021 May 5.
A new member of the genus Closterovirus was detected in Platycodon grandiflorus using high-throughput RNA sequencing analysis. The complete genome sequence of this new virus isolate, tentatively named "platycodon closterovirus 1" (PlaCV1), comprises 16,771 nucleotides with nine predicted open reading frames (ORFs) having the typical closterovirus genome organization. PlaCV1 shares 37%-50% nucleotide sequence identity with other known closterovirus genome sequences. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70-like protein (HSP70h), viral heat shock protein 90-like protein (HSP90h), minor coat protein (CPm), and coat protein (CP) show 47-68%, 39-66%, 24-52%, 21-57%, and 16-35% amino acid sequence identity, respectively, to homologous proteins in previously identified closteroviruses, suggesting that it represents a distinct, new species in the genus. Phylogenetic analysis of HSP70h sequences places PlaCV1 alongside other members of the genus Closterovirus in the family Closteroviridae. To our knowledge, this study is the first report of the complete genome sequence of PlaCV1 infecting P. grandiflorus in the Republic of Korea.
利用高通量 RNA 测序分析，在桔梗中检测到一个新的 Closterovirus 属成员。这种新病毒分离株的完整基因组序列，暂定名为“桔梗环斑病毒 1”（PlaCV1），由 16771 个核苷酸组成，具有九个预测的开放阅读框（ORFs），具有典型的环斑病毒基因组结构。PlaCV1 与其他已知的环斑病毒基因组序列共享 37%-50%的核苷酸序列同一性。推定的 RNA 依赖性 RNA 聚合酶（RdRp）、热休克蛋白 70 样蛋白（HSP70h）、病毒热休克蛋白 90 样蛋白（HSP90h）、次要衣壳蛋白（CPm）和衣壳蛋白（CP）分别与先前鉴定的环斑病毒中的同源蛋白具有 47-68%、39-66%、24-52%、21-57%和 16-35%的氨基酸序列同一性，表明它代表了该属中的一个独特的新种。HSP70h 序列的系统发育分析将 PlaCV1 与家族 Closteroviridae 中的其他 Closterovirus 属成员放在一起。据我们所知，这是首次报道 PlaCV1 感染韩国桔梗的完整基因组序列。
