Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea.
Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
Arch Virol. 2022 Jun;167(6):1491-1494. doi: 10.1007/s00705-022-05448-0. Epub 2022 Apr 26.
The genome of a novel virus identified in Cnidium officinale is composed of a monopartite ssRNA of 16,755 nucleotides that shares 68.73% (query coverage, 20%) sequence identity with carrot yellow leaf virus (CYLV, accession no. FJ869862.1). It contains 11 putative open reading frames and has an organization typical of closteroviruses. It shares 30-50% nucleotide sequence identity with other closteroviruses. The heat shock protein 70-like protein (HSP70), putative RNA-dependent RNA polymerase (RdRp), and coat protein (CP) show 39-66%, 16-60%, and 24-41% amino acid sequence identity, respectively, to the homologous proteins of previously identified closteroviruses. Molecular and HSP70-based phylogenetic analysis of the genome and encoded protein sequences suggested that this virus is a novel member of the genus Closterovirus in the family Closteroviridae, which we have tentatively named "cnidium closterovirus 1" (CnClV1).
从蛇床中鉴定出的一种新型病毒的基因组由单链 RNA 组成，其全长为 16755 个核苷酸，与胡萝卜黄化病毒（CYLV，登录号 FJ869862.1）的序列同源性为 68.73%（查询覆盖率 20%）。它包含 11 个推定的开放阅读框，具有典型的杆状病毒结构。它与其他杆状病毒的核苷酸序列同源性为 30-50%。热休克蛋白 70 样蛋白（HSP70）、推定的 RNA 依赖性 RNA 聚合酶（RdRp）和外壳蛋白（CP）与先前鉴定的杆状病毒同源蛋白的氨基酸序列同源性分别为 39-66%、16-60%和 24-41%。基于基因组和编码蛋白序列的分子和 HSP70 系统发育分析表明，该病毒是杆状病毒科杆状病毒属的一个新成员，我们暂定名为“蛇床子杆状病毒 1”（CnClV1）。
