Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, Yunnan, 650201, China.
Arch Virol. 2023 May 5;168(5):154. doi: 10.1007/s00705-023-05775-w.
The complete genome sequence of a putative novel closterovirus, tentatively named "Dregea volubilis closterovirus 1" (DvCV1, GenBank accession no. MZ779122), infecting Dregea volubilis in China was determined using high-throughput sequencing (HTS). The complete genome sequence of DvCV1 consists of 16,165 nucleotides (nt) and contains nine ORFs. The genome structure of DvCV1 is typical of members of the genus Closterovirus. Complete genome sequence analysis showed that DvCV1 shares 41.4-48.4% nucleotide sequence identity with other known closteroviruses. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70-like protein (HSP70h), and coat protein (CP) of DvCV1 share 46.80-62.65%, 31.06-51.80%, and 28.34-37.37% amino acid sequence identity, respectively, with the RdRp, HSP70h and CP of other closteroviruses. Phylogenetic analysis based on HSP70h aa sequences placed DvCV1 alongside other members of the genus Closterovirus in the family Closteroviridae. These results suggest that DvCV1 is a new member of the genus Closterovirus. This is the first report of a closterovirus infecting D. volubilis.
一株疑似新的杆状病毒——暂定名为“卷丹潜隐病毒 1 号”（Dregea volubilis closterovirus 1，DvCV1，GenBank 登录号 MZ779122）的全基因组序列，通过高通量测序（HTS）被测定。DvCV1 的全基因组序列由 16165 个核苷酸（nt）组成，包含 9 个 ORF。DvCV1 的基因组结构是典型的杆状病毒属成员。完整的基因组序列分析表明，DvCV1 与其他已知的杆状病毒共享 41.4-48.4%的核苷酸序列同一性。DvCV1 的推定 RNA 依赖性 RNA 聚合酶（RdRp）、热休克蛋白 70 样蛋白（HSP70h）和外壳蛋白（CP）分别与其他杆状病毒的 RdRp、HSP70h 和 CP 共享 46.80-62.65%、31.06-51.80%和 28.34-37.37%的氨基酸序列同一性。基于 HSP70h aa 序列的系统发育分析将 DvCV1 与杆状病毒属的其他成员一起置于杆状病毒科中。这些结果表明，DvCV1 是杆状病毒属的一个新成员。这是首例报道的感染卷丹的杆状病毒。
