二维凝胶在定量蛋白质组学中的位置。

The Whereabouts of 2D Gels in Quantitative Proteomics.

机构信息

Chemistry and Biology of Metals, Univ. Grenoble-Alpes, CNRS UMR5249, IRIG-DIESE-CBM, CEA Grenoble, Grenoble Cedex 9, France.

出版信息

Methods Mol Biol. 2021;2228:41-51. doi: 10.1007/978-1-0716-1024-4_4.

DOI:10.1007/978-1-0716-1024-4_4

PMID:33950482

Abstract

Two-dimensional gel electrophoresis has been instrumental in the development of proteomics. Although it is no longer the exclusive scheme used for proteomics, its unique features make it a still highly valuable tool, especially when multiple quantitative comparisons of samples must be made, and even for large samples series. However, quantitative proteomics using two-dimensional gels is critically dependent on the performances of the protein detection methods used after the electrophoretic separations. This chapter therefore examines critically the various detection methods, (radioactivity, dyes, fluorescence, and silver) as well as the data analysis issues that must be taken into account when quantitative comparative analysis of two-dimensional gels is performed.

摘要

二维凝胶电泳在蛋白质组学的发展中具有重要作用。尽管它不再是蛋白质组学唯一使用的方案，但它独特的特点使其仍然是一种非常有价值的工具，特别是当需要对多个样品进行定量比较时，甚至对于大型样品系列也是如此。然而，使用二维凝胶进行定量蛋白质组学在很大程度上取决于电泳分离后使用的蛋白质检测方法的性能。因此，本章批判性地研究了各种检测方法（放射性、染料、荧光和银染）以及在进行二维凝胶定量比较分析时必须考虑的数据分析问题。