Koiwai O, Morita A
Institute for Developmental Research, Aichi Prefectural Colony, Japan.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):91-100. doi: 10.1016/0006-291x(88)90654-7.
We have isolated the genomic clone, which contained 5'-flanking region for human terminal deoxynucleotidyltransferase (TdT) gene, by screening Charon 4A library and determined its nucleotide sequence. Two transcription initiation sites (cap sites) were localized at 81 and 106 bp upstream from the third ATG codon which is translation initiation site, by primer extension analysis. Although neither typical TATA nor CAAT sequences were detected within 100 bp upstream region of the 5' cap site, a palindromic structure comprised of 28 bp was formed just upstream of the 5' cap site and double repeats of 5'-CTGGC sequence were found in its palindrom. The typical octamer of 5'-ATGCAAAT and three octamer-like sequences, which are involved in the transcription of lymphoid-specific genes, were detected at 212, 293, 347 and 770 bp upstream from the first cap site.
我们通过筛选Charon 4A文库分离出了基因组克隆，该克隆包含人类末端脱氧核苷酸转移酶（TdT）基因的5'侧翼区域，并确定了其核苷酸序列。通过引物延伸分析，两个转录起始位点（帽位点）位于第三个ATG密码子（即翻译起始位点）上游81和106 bp处。尽管在5'帽位点上游100 bp区域内未检测到典型的TATA或CAAT序列，但在5'帽位点上游刚好形成了一个由28 bp组成的回文结构，并且在其回文中发现了5'-CTGGC序列的双重重复。在第一个帽位点上游212、293、347和770 bp处检测到了5'-ATGCAAAT的典型八聚体和三个与淋巴特异性基因转录有关的类八聚体序列。
