Koiwai O, Hasada K, Yasui Y, Sakai Y, Sato H, Watanabe T
Department of Biochemistry, Aichi Cancer Center Institute, Nagoya, Japan.
Biochem Genet. 1995 Apr;33(3-4):111-22. doi: 10.1007/BF00557949.
The mouse gene for phenol UDP-glucuronosyltransferase (UDPGT; Ugtla1) was mapped at 42 cM on chromosome 1, a position identical to that of the gene for bilirubin UDPGT (Ugtla1), from linkage analysis of a three-point cross test with Idh-1, En-1, and Ugtla1 as marker genes. The cDNAs for mouse phenol and bilirubin UDPGTs, isolated after amplification by PCR, shared an identical 3'-half region. Our results strongly suggest that mouse bilirubin and phenol UDPGTs are expressed from a single gene and involve alternative splicing events. We also detected duplication of the gene for phenol UDPGT in all mouse strains examined with the exception of MOL-MIT and SUB-SHH.
通过以异柠檬酸脱氢酶-1（Idh-1）、烯醇酶-1（En-1）和尿苷二磷酸葡萄糖醛酸基转移酶1（Ugtla1）作为标记基因进行三点杂交试验的连锁分析，发现小鼠酚类尿苷二磷酸葡萄糖醛酸基转移酶（UDPGT；Ugtla1）基因定位于1号染色体上42厘摩处，该位置与胆红素UDPGT（Ugtla1）基因相同。经聚合酶链反应（PCR）扩增后分离得到的小鼠酚类和胆红素UDPGT的互补DNA（cDNA），共享一个相同的3'端半区。我们的结果强烈表明，小鼠胆红素和酚类UDPGT由单一基因表达，并涉及可变剪接事件。我们还在除MOL-MIT和SUB-SHH之外的所有检测小鼠品系中检测到酚类UDPGT基因的重复。
