长链非编码 RNA-微小 RNA-mRNA 轴在肌萎缩侧索硬化症中的新调控作用：整合生物信息学分析。

The Novel Regulatory Role of lncRNA-miRNA-mRNA Axis in Amyotrophic Lateral Sclerosis: An Integrated Bioinformatics Analysis.

机构信息

Department of Oncology and Hematology, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, China.

Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai, China.

出版信息

Comput Math Methods Med. 2021 Apr 15;2021:5526179. doi: 10.1155/2021/5526179. eCollection 2021.

Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease that primarily affects motor neurons, causing muscle atrophy, bulbar palsy, and pyramidal tract signs. However, the aetiology and pathogenesis of ALS have not been elucidated to date. In this study, a competitive endogenous RNA (ceRNA) network was constructed by analyzing the expression profiles of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) that were matched by 7 ALS samples and 4 control samples, and then a protein-protein interaction (PPI) network was constructed to identify the genes related to ALS. Gene Ontology (GO) was used to study the potential functions of differentially expressed mRNAs (DEmRNAs) in the ceRNA network. For the ALS and control groups, 247177 potential lncRNA-mRNA ceRNA relationship pairs were screened. Analysis of significant relationship pairs demonstrated that the PPI modules formed by the -regulated , , , , and genes may play important roles in the pathogenesis of ALS, and these results may help to characterize the pathogenesis of ALS.

肌萎缩侧索硬化症（ALS）是一种不可治愈的神经退行性疾病，主要影响运动神经元，导致肌肉萎缩、延髓性麻痹和锥体束征。然而，ALS 的病因和发病机制迄今尚未阐明。本研究通过分析 7 例 ALS 样本和 4 例对照样本匹配的信使 RNA（mRNA）和长链非编码 RNA（lncRNA）的表达谱，构建了竞争性内源 RNA（ceRNA）网络，然后构建了蛋白质-蛋白质相互作用（PPI）网络，以确定与 ALS 相关的基因。GO 用于研究 ceRNA 网络中差异表达 mRNAs（DEmRNAs）的潜在功能。对于 ALS 和对照组，筛选出 247177 对潜在的 lncRNA-mRNA ceRNA 关系对。对显著关系对的分析表明，由-调控的、、、、和基因形成的 PPI 模块可能在 ALS 的发病机制中发挥重要作用，这些结果可能有助于表征 ALS 的发病机制。