Sherzad Raoof Hana, Baba Sheikh Mohammed Omar, Sulaiman Rizgar Raheem
Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq.
Veterinary Laboratory, PCR Unit, Directorate of Veterinary, Sulaimani, Sulaymaniyah, Iraq.
Vet Res Forum. 2021 Winter;12(1):117-120. doi: 10.30466/vrf.2018.94774.2281. Epub 2021 Mar 15.
Infectious Bronchitis (IB) is one of the most important viral diseases of poultry. It causes major financial losses in the poultry industry. It is caused by the infectious bronchitis virus (IBV). This study investigated the genetic origin and diversity of IBV by analyzing the partial S1 sequence derived from local broiler farms in different regions of Sulaimani Governorate. Detection was performed using a newly designed primer for the partial S1 gene. The present was undertaken to understand the molecular relationship between the strains across the world compared with field virus isolates by phylogenetic tree and sequences-analysis. Phylogenetic tree demonstrated that field isolate made a novel group (sul-5/17-like strain), indicating new variants. Also, field virus sequences were closely matched to Iranian strain HQ123347/RFIBV6 (97.00%). Further sequences analysis exhibited that field isolate was not homologous but high divergences with reverence vaccines strains. These results indicated that differences in S1 protein of the locally circulated IBV could be a reason for currents vaccination failure.
传染性支气管炎（IB）是家禽最重要的病毒性疾病之一。它给家禽业造成重大经济损失。该病由传染性支气管炎病毒（IBV）引起。本研究通过分析来自苏莱曼尼亚省不同地区当地肉鸡场的部分S1序列，调查了IBV的基因起源和多样性。使用新设计的针对部分S1基因的引物进行检测。本研究旨在通过系统发育树和序列分析，了解与田间病毒分离株相比，世界各地毒株之间的分子关系。系统发育树表明，田间分离株形成了一个新的群体（sul-5/17样毒株），表明存在新的变异体。此外，田间病毒序列与伊朗毒株HQ123347/RFIBV6密切匹配（97.00%）。进一步的序列分析表明，田间分离株与参考疫苗株不同源，但差异很大。这些结果表明，当地流行的IBV的S1蛋白差异可能是当前疫苗接种失败的一个原因。
