Modiri Hamadan Amir, Ghalyanchilangeroudi Arash, Hashemzadeh Masoud, Hosseini Hossein, Karimi Vahid, Yahyaraeyat Ramak, Najafi Hamideh
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Virus Res. 2017 Aug 15;240:101-106. doi: 10.1016/j.virusres.2017.08.002. Epub 2017 Aug 5.
Avian infectious bronchitis virus (IBV) is causing major economic losses to the poultry industry. The analysis of the S1 gene has been used to determine IBV genotype. The aim of this study was genotyping of IBVs circulating among the Iranian broiler flocks in the period between 2015 to 2017. Trachea samples from 278 broiler flocks were collected from broiler farms in eight provinces of Iran. After Real-time RT-PCR, IBV-positive samples were further characterized based on S1 gene. The results of the Real-time RT-PCR showed that 52.16% of flocks were IBV positive. Four genotypes were detected and the frequency of occurrence rates of IS-1494-like, 793/B, QX and Massachusetts IBV genotypes were 70.34%, 19.31%, 7.58% and 2.75%, respectively. Sequence analysis revealed that nucleotide identities within IS-1494-like group ranged between 98.86-100%, while each of the QX, Massachusetts and 793/B groups were 98.05-100%, 98.20-100% and 93.29-100% respectively. These results show that the IS-1494-like IBV is the dominant IBV genotype in Iran. Proper control strategies are essential to overcoming the high frequency of occurrence of IS-1494-like IBV. The phylogenetic relationship of the strains with respect to different sequences and geographical regions displayed complexity and diversity. Further studies are needed and should include the isolation and full-length molecular characterization of IBV in Iran.
禽传染性支气管炎病毒（IBV）给家禽业造成了重大经济损失。对S1基因的分析已被用于确定IBV基因型。本研究的目的是对2015年至2017年期间在伊朗肉鸡群中传播的IBV进行基因分型。从伊朗八个省份的肉鸡养殖场收集了278个肉鸡群的气管样本。经过实时RT-PCR检测后，对IBV阳性样本基于S1基因进行进一步鉴定。实时RT-PCR结果显示，52.16%的鸡群为IBV阳性。检测到四种基因型，IS-1494-like、793/B、QX和马萨诸塞州IBV基因型的出现频率分别为70.34%、19.31%、7.58%和2.75%。序列分析表明，IS-1494-like组内的核苷酸同一性在98.86 - 100%之间，而QX、马萨诸塞州和793/B组分别为98.05 - 100%、98.20 - 100%和93.29 - 100%。这些结果表明，IS-1494-like IBV是伊朗主要的IBV基因型。适当的控制策略对于克服IS-1494-like IBV的高发生率至关重要。菌株在不同序列和地理区域方面的系统发育关系显示出复杂性和多样性。需要进一步研究，且应包括伊朗IBV的分离和全长分子特征分析。
