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养殖大菱鲆（Psetta maxima）幼体中海洋尾丝虫（原生动物，纤毛虫纲，盾纤目）的分子鉴定

Molecular identification of Uronema marinum (Protozoa, Ciliophora, Scuticociliatia) in cultured turbot (Psetta maxima) larvae.

作者信息

Türe Mustafa

机构信息

Department of Fisheries Health, Central Fisheries Research Institute, Trabzon, Turkey .

出版信息

Vet Res Forum. 2021 Winter;12(1):121-124. doi: 10.30466/vrf.2020.110220.2614. Epub 2021 Mar 15.

DOI:10.30466/vrf.2020.110220.2614
PMID:33953883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8094146/
Abstract

Scuticociliates are dangerous parasitic pathogens causing systemic tissue destruction and high mortality in marine fish worldwide. In this study, the first identification of (Ciliophora, Scuticociliatida) from cultured turbot () larvae using mitochondrial cytochrome c oxidase 1 (1) gene sequence as well as species-specific primers was reported. The mean prevalence values of infected fish were calculated, and partial sequencing obtained from the mitochondrial 1 gene region was also compared with isolates registered in the Genbank database. The sequence comparison showed 93.00% identity to , and the parasite has also been deposited in the GenBank database. This study is the first case of infection in Turkish marine aquaculture, contributing to the systematics and molecular epidemiology of scuticociliate in Turkey.

摘要

盾纤毛虫是危险的寄生性病原体，在全球范围内会导致海水鱼类的系统性组织破坏和高死亡率。在本研究中，报告了首次利用线粒体细胞色素c氧化酶1（CO1）基因序列以及物种特异性引物，从养殖大菱鲆（Scophthalmus maximus）幼体中鉴定出（纤毛虫纲，盾纤目）。计算了感染鱼的平均患病率，并将从线粒体CO1基因区域获得的部分序列与Genbank数据库中登记的分离株进行了比较。序列比较显示与[某物种]有93.00%的同一性，该寄生虫也已存入GenBank数据库。本研究是土耳其海水养殖中[该寄生虫名称]感染的首例，为土耳其盾纤毛虫的系统学和分子流行病学研究做出了贡献。

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本文引用的文献

1
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.MEGA X：跨越计算平台的分子进化遗传学分析。
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. doi: 10.1093/molbev/msy096.
2
Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa.纤毛原生动物分类学研究的基本光镜和扫描电镜方法。
Eur J Protistol. 1991 Nov 29;27(4):313-30. doi: 10.1016/S0932-4739(11)80248-8. Epub 2011 Nov 2.
3
Identification of scuticociliates (Pseudocohnilembus persalinus, P. longisetus, Uronema marinum and Miamiensis avidus) based on the cox1 sequence.
基于细胞色素氧化酶亚基I（cox1）序列鉴定盾纤毛虫（海水伪康纤虫、长伪康纤虫、海生尾丝虫和嗜热迈阿密虫）
Parasitol Int. 2013 Feb;62(1):7-13. doi: 10.1016/j.parint.2012.08.002. Epub 2012 Aug 19.
4
Small subunit ribosomal RNA and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate Miamiensis avidus (Ciliophora, Scuticociliatia).21 株寄生旋口虫 Miamiensis avidus（纤毛门，旋口目）的小亚基核糖体 RNA 和线粒体细胞色素 c 氧化酶亚基 1 基因序列。
Parasitol Res. 2011 May;108(5):1153-61. doi: 10.1007/s00436-010-2157-7. Epub 2010 Nov 27.
5
A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood.一种通过最大似然法估计大型系统发育树的简单、快速且准确的算法。
Syst Biol. 2003 Oct;52(5):696-704. doi: 10.1080/10635150390235520.
6
Philasterides dicentrarchi (Ciliophora, Scuticociliatida) as the causative agent of scuticociliatosis in farmed turbot Scophthalmus maximus in Galicia (NW Spain).
Dis Aquat Organ. 2001 Aug 22;46(1):47-55. doi: 10.3354/dao046047.
7
Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees.人类和黑猩猩线粒体DNA控制区域核苷酸替换数目的估计。
Mol Biol Evol. 1993 May;10(3):512-26. doi: 10.1093/oxfordjournals.molbev.a040023.
8
DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.用于从多种后生动物无脊椎动物中扩增线粒体细胞色素c氧化酶亚基I的DNA引物。
Mol Mar Biol Biotechnol. 1994 Oct;3(5):294-9.