DeLaurier April, Howe Douglas G, Ruzicka Leyla, Carte Adam N, Mishoe Hernandez Lacie, Wiggins Kali J, Gallati Mika M, Vanpelt Kayce, Loyo Rosado Frances, Pugh Katlin G, Shabdue Chasey J, Jihad Khadijah, Thyme Summer B, Talbot Jared C
Department of Biology and Geology, University of South Carolina -Aiken, Aiken, SC, United States of America.
The Institute of Neuroscience, University of Oregon, Eugene, OR, United States of America.
PeerJ. 2021 Apr 13;9:e11007. doi: 10.7717/peerj.11007. eCollection 2021.
In the past decade, the zebrafish community has widely embraced targeted mutagenesis technologies, resulting in an abundance of mutant lines. While many lines have proven to be useful for investigating gene function, many have also shown no apparent phenotype, or phenotypes not of interest to the originating lab. In order for labs to document and share information about these lines, we have created ZebraShare as a new resource offered within ZFIN.
ZebraShare involves a form-based submission process generated by ZFIN. The ZebraShare interface (https://zfin.org/action/zebrashare) can be accessed on ZFIN under "Submit Data". Users download the Submission Workbook and complete the required fields, then submit the completed workbook with associated images and captions, generating a new ZFIN publication record. ZFIN curators add the submitted phenotype and mutant information to the ZFIN database, provide mapping information about mutations, and cross reference this information across the appropriate ZFIN databases. We present here examples of ZebraShare submissions, including and mutant lines.
Users can find ZebraShare submissions by searching ZFIN for specific alleles or line designations, just as for alleles submitted through the normal process. We present several potential examples of submission types to ZebraShare including a phenotypic mutants, mildly phenotypic, and early lethal mutants. Mutants for show no apparent skeletal phenotype, and mutants show only a mild skeletal phenotype, yet these genes have specific human disease relevance and therefore may be useful for further studies. The p120-catenin encoding gene, was knocked out to investigate a potential role in brain development or function. The homozygous mutant disintegrates during early somitogenesis and the heterozygote has localized defects, revealing vital roles in early development. Two genes were knocked out to investigate a role in muscle formation. The double mutant has an early embryonic lethal phenotype, potentially related to a proposed role in the core splicing complex. In each example, the mutants submitted to ZebraShare display phenotypes that are not ideally suited to their originating lab's project directions but may be of great relevance to other researchers.
ZebraShare provides an opportunity for researchers to directly share information about mutant lines within ZFIN, which is widely used by the community as a central database of information about zebrafish lines. Submissions of alleles with a phenotypic or unexpected phenotypes is encouraged to promote collaborations, disseminate lines, reduce redundancy of effort and to promote efficient use of time and resources. We anticipate that as submissions to ZebraShare increase, they will help build an ultimately more complete picture of zebrafish genetics and development.
在过去十年中，斑马鱼研究群体广泛采用了靶向诱变技术，产生了大量突变品系。虽然许多品系已被证明对研究基因功能有用，但也有许多品系未表现出明显的表型，或者表现出的表型并非原始实验室感兴趣的。为了让实验室记录并分享有关这些品系的信息，我们创建了ZebraShare，作为ZFIN提供的一项新资源。
ZebraShare涉及由ZFIN生成的基于表单的提交过程。ZebraShare界面（https://zfin.org/action/zebrashare）可在ZFIN的“提交数据”下访问。用户下载提交工作簿并填写所需字段，然后提交带有相关图像和说明的已完成工作簿，生成一条新的ZFIN出版物记录。ZFIN管理员将提交的表型和突变信息添加到ZFIN数据库中，提供有关突变的定位信息，并在适当的ZFIN数据库中交叉引用此信息。我们在此展示ZebraShare提交内容的示例，包括 和 突变品系。
用户可以通过在ZFIN中搜索特定等位基因或品系名称来查找ZebraShare提交内容，就像查找通过正常流程提交的等位基因一样。我们展示了向ZebraShare提交的几种潜在类型的示例，包括表型突变体、轻度表型突变体和早期致死突变体。 的突变体未表现出明显的骨骼表型，而 的突变体仅表现出轻度的骨骼表型，但这些基因与特定的人类疾病相关，因此可能对进一步研究有用。敲除了编码p120连环蛋白的基因 ，以研究其在脑发育或功能中的潜在作用。纯合 突变体在早期体节形成过程中解体，杂合子有局部缺陷，揭示了其在早期发育中的重要作用。敲除了两个 基因，以研究其在肌肉形成中的作用。 双突变体具有早期胚胎致死表型，可能与在核心剪接复合体中的假定作用有关。在每个示例中，提交到ZebraShare的突变体所表现出的表型并不完全符合其原始实验室的项目方向，但可能对其他研究人员具有重要意义。
ZebraShare为研究人员提供了一个在ZFIN内直接分享突变品系信息的机会，ZFIN被该群体广泛用作斑马鱼品系信息的中央数据库。鼓励提交具有表型或意外表型的等位基因，以促进合作、传播品系、减少工作冗余，并促进时间和资源的有效利用。我们预计，随着向ZebraShare提交的内容增加，它们将有助于构建一个最终更完整的斑马鱼遗传学和发育图景。
