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通过DNA-DNA杂交和细胞脂肪酸分析对副结核分枝杆菌临床分离株进行鉴定

Characterization of clinical isolates of Mycobacterium paratuberculosis by DNA-DNA hybridization and cellular fatty acid analysis.

作者信息

Saxegaard F, Baess I, Jantzen E

机构信息

National Veterinary Institute, Oslo, Norway.

出版信息

APMIS. 1988 Jun;96(6):497-502.

PMID:3395476
Abstract

The DNA-DNA homologies obtained were more than 90 per cent for all strains examined, including the reference strains of Mycobacterium paratuberculosis and Mycobacterium avium. Consequently, in a genetic sense, M. paratuberculosis with its variants belong to a single species which should be considered a subspecies of M. avium. The same reference strains of M. paratuberculosis and M. avium showed small but distinct differences in the cellular fatty acid patterns. The Norwegian goat isolate proved to be M. paratuberculosis, while the three sheep isolates from Iceland and the Faroe Islands showed a typical M. avium pattern.

摘要

对所有检测菌株,包括副结核分枝杆菌和鸟分枝杆菌的参考菌株,所获得的DNA-DNA同源性均超过90%。因此,从遗传学意义上讲,副结核分枝杆菌及其变种属于单一物种,应被视为鸟分枝杆菌的一个亚种。副结核分枝杆菌和鸟分枝杆菌的相同参考菌株在细胞脂肪酸模式上显示出微小但明显的差异。挪威山羊分离株被证明是副结核分枝杆菌,而来自冰岛和法罗群岛的三只绵羊分离株显示出典型的鸟分枝杆菌模式。

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