Lévy-Frébault V V, Thorel M F, Varnerot A, Gicquel B
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
J Clin Microbiol. 1989 Dec;27(12):2823-6. doi: 10.1128/jcm.27.12.2823-2826.1989.
Mycobacterium paratuberculosis strains, mycobacteria from patients suffering from Crohn's disease, "wood pigeon mycobacteria," and representatives of Mycobacterium avium-Mycobacterium intracellulare were compared by restriction endonuclease DraI digestion and field inversion gel electrophoresis. Characteristic profiles were seen for M. paratuberculosis, including isolates from patients suffering from Crohn's disease, for wood pigeon mycobacteria, and for M. avium-M. intracellulare serotypes 2, 16, 18, and 19. Two M. paratuberculosis strains used for vaccine production (St 18 and 316 F) presented patterns different from those of the other M. paratuberculosis strains. Strains St 18 yielded a pattern identical to that of the M. avium type strain serotype 2, whereas 316 F gave a unique pattern. The method developed in this study represents a useful taxonomic tool for the identification and classification of mycobacteria.
通过限制性内切酶DraI消化和场反转凝胶电泳，对副结核分枝杆菌菌株、克罗恩病患者体内的分枝杆菌、“林鸽分枝杆菌”以及鸟分枝杆菌-胞内分枝杆菌的代表菌株进行了比较。副结核分枝杆菌呈现出特征性图谱，包括来自克罗恩病患者的分离株、林鸽分枝杆菌以及鸟分枝杆菌-胞内分枝杆菌血清型2、16、18和19。用于疫苗生产的两株副结核分枝杆菌（St 18和316 F）呈现出与其他副结核分枝杆菌菌株不同的图谱。菌株St 18产生的图谱与鸟分枝杆菌血清型2的模式菌株相同，而316 F则呈现出独特的图谱。本研究中开发的方法是一种用于分枝杆菌鉴定和分类的有用的分类工具。
