Franzén L, Norrby K
Department of Pathology, University of Linköping, Sweden.
APMIS. 1988 Jun;96(6):519-24. doi: 10.1111/j.1699-0463.1988.tb05338.x.
The healing of perforated mesenterial window is delayed in insulin-deficient rats after 4 weeks of streptozoticin-induced diabetes. In the present study we investigated the mitogenic capacity of the two predominating cell types in the healing mesenterial window in such rats. The labelling index (LI) in fibroblasts and mesothelial cells, normally constituting approximately 96% of all tissue-bound cells, was estimated in early and later phases of healing within (a) a 1 mm-wide zone surrounding the perforation and (b) centrally in wounds after healing by closure. The mitotic index (MI) of these cells was estimated at various distances from the perforation. Adjacent unperforated control mesenteric windows served as internal controls. Proliferation increased on days 1 and 2 post-perforation, whereafter it gradually diminished, fibroblasts showing a higher LI than mesothelial cells days 3-7 after closure. On day 1 post-perforation the relative increase of LI was greatest in diabetic mesenteries. During the period just preceding healing by closure. LI of both fibroblasts and mesothelial cells was, however, significantly reduced in diabetic animals. The impaired mitogenesis in these wound-healing cells in diabetic rats may thus be of pathogenic significance in the delayed healing in such animals.
在链脲佐菌素诱导糖尿病4周后的胰岛素缺乏大鼠中，肠系膜窗穿孔的愈合延迟。在本研究中，我们调查了此类大鼠愈合中的肠系膜窗中两种主要细胞类型的促有丝分裂能力。在愈合的早期和后期阶段，在（a）围绕穿孔的1毫米宽区域以及（b）愈合后伤口中央，估计通常占所有组织结合细胞约96%的成纤维细胞和间皮细胞的标记指数（LI）。在距穿孔不同距离处估计这些细胞的有丝分裂指数（MI）。相邻的未穿孔对照肠系膜窗用作内部对照。穿孔后第1天和第2天增殖增加，此后逐渐减少，成纤维细胞在闭合后第3 - 7天显示出比间皮细胞更高的LI。穿孔后第1天，糖尿病肠系膜中LI的相对增加最大。然而，在通过闭合愈合之前的时期，糖尿病动物中成纤维细胞和间皮细胞的LI均显著降低。因此，糖尿病大鼠这些伤口愈合细胞中有丝分裂的受损可能对此类动物愈合延迟具有致病意义。
