Franzén L, Ghassemifar R, Malcherek P
Department of Pathology II, University Hospital, Linköping, Sweden.
Agents Actions. 1991 Jul;33(3-4):371-7. doi: 10.1007/BF01986588.
The participation of mast cells in connective tissue repair was studied using the perforated-rat-mesentery model. Perforation of mesenteric membranes was performed during laparotomy of anesthetized Sprague-Dawley rats. Laparotomy significantly reduced the histamine content of the mesenteric membranes on day 1 postoperatively and perforation as such reduced the histamine content even more on days 1-10. Mast cell activation induced by a single intraperitoneal injection of Compound 48/80 two days prior to operation, significantly improved healing on days 5-7 postoperatively. Daily injections of Compound 48/80 for 5 days prior to operation showed significantly better healing compared to such injections for five days postoperatively. Administration of lupitidine, a long acting histamine H2-receptor antagonist, two times daily starting on the day of 48/80 injection to day 4 after operation did not apparently affect healing. The results indicate that mast cells may be activated during normal wound healing and that a preoperative, pharmacological activation improves healing. Furthermore, histamine does not seem to be of major importance for the beneficial effect of such mast cell activation on connective tissue repair.
利用大鼠肠系膜穿孔模型研究了肥大细胞在结缔组织修复中的作用。在麻醉的斯普拉格-道利大鼠剖腹手术期间进行肠系膜膜穿孔。剖腹手术在术后第1天显著降低了肠系膜膜中的组胺含量，而穿孔本身在术后1至10天进一步降低了组胺含量。术前两天单次腹腔注射化合物48/80诱导的肥大细胞活化，在术后第5至7天显著促进了愈合。术前连续5天每日注射化合物48/80，与术后连续5天注射相比，愈合效果明显更好。从注射48/80当天开始至术后第4天，每天两次给予长效组胺H2受体拮抗剂鲁匹替丁，对愈合没有明显影响。结果表明，肥大细胞可能在正常伤口愈合过程中被激活，术前的药物激活可促进愈合。此外，组胺似乎对肥大细胞激活对结缔组织修复的有益作用并不重要。
