Franzén L, Adamson P, Norrby K
Department of Pathology, Universities of Linköping, Sweden.
Int J Microcirc Clin Exp. 1990 Nov;9(4):401-10.
The neovascularization that occurs during wound healing in the perforated mesenteric membrane was quantitatively studied in rats with streptozotocin induced diabetes. The virtually avascular mesenteric windows in both controls and diabetic rats were perforated with a scalpel during laparatomy. At designated intervals during days 1-63 postoperatively, the mesenteries were excised, fixed, and cut perpendicularly through the wound margin or the central part of a closed wound. An unperforated window from each animal served as an internal control. The numerical microvascular density of the healing tissue and the unperforated control mesentery was assessed by morphometry on photomicrographs with regard to number of vascular profiles per mm length of mesentery or mm2 sectioned tissue area. The vascularity in unperforated windows of both diabetics and age-matched healthy controls was unaffected by laparotomy whereas perforation significantly increased the numerical microvascular density of the mesentery. The onset of angiogenesis in the perforated mesenteries occurred on days 5-7 and in both diabetics and controls, wound areas retained an increased numerical microvascular density throughout the observation period. The numerical microvascular density of the wound areas was greater in diabetics than controls following closure of the wounds. A number of wounds healed without being vascularized. The number of vascularized wounds was significantly higher in diabetic animals than controls.
在链脲佐菌素诱导的糖尿病大鼠中，对穿孔肠系膜膜伤口愈合过程中发生的新生血管形成进行了定量研究。在剖腹手术期间，用手术刀在对照组和糖尿病大鼠几乎无血管的肠系膜窗口上穿孔。在术后1 - 63天的指定时间间隔，切除肠系膜，固定，并垂直于伤口边缘或闭合伤口的中心部分进行切割。每只动物的一个未穿孔窗口作为内部对照。通过形态计量学在显微照片上评估愈合组织和未穿孔对照肠系膜的微血管密度，以每毫米肠系膜长度或每平方毫米切片组织面积的血管轮廓数量来衡量。糖尿病患者和年龄匹配的健康对照的未穿孔窗口中的血管形成不受剖腹手术的影响，而穿孔显著增加了肠系膜的微血管密度。穿孔肠系膜中血管生成的起始发生在第5 - 7天，在糖尿病患者和对照组中，伤口区域在整个观察期内微血管密度均保持增加。伤口闭合后，糖尿病患者伤口区域的微血管密度高于对照组。许多伤口在没有血管化的情况下愈合。糖尿病动物中血管化伤口的数量显著高于对照组。
