Lin Meei-Hua, Miner Jeffrey H, Hsu Fong-Fu
Division of Nephrology, Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO, USA.
Methods Mol Biol. 2021;2306:139-155. doi: 10.1007/978-1-0716-1410-5_10.
Lipidomic analyses by mass spectrometry (MS) of epidermal ceramides, a large family of lipids crucial to the permeability barrier of the skin, have been reported previously. To ensure the accuracy of lipid identification, we describe here the isolation of mouse newborn epidermal lipids followed by fractionation with solid-phase extraction columns, and lipidomic analyses by high-resolution MS for structural identification. We also describe here the employment of thin layer chromatography, an old but useful tool, in facilitating the structural characterization of the epidermal lipid species by MS.
此前已有报道通过质谱（MS）对表皮神经酰胺进行脂质组学分析，表皮神经酰胺是对皮肤渗透屏障至关重要的一大类脂质。为确保脂质鉴定的准确性，我们在此描述从小鼠新生表皮中分离脂质，随后使用固相萃取柱进行分离，以及通过高分辨率质谱进行脂质组学分析以进行结构鉴定的方法。我们还在此描述使用薄层色谱法（一种古老但有用的工具）来促进通过质谱对表皮脂质种类进行结构表征。
