Department of Chemistry, Università di Roma "La Sapienza", Rome, Italy.
Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
Methods Mol Biol. 2021;2306:257-273. doi: 10.1007/978-1-0716-1410-5_17.
The chemical composition of Cannabis sativa L. has been extensively studied for tens of years, but little is known about its lipidome. This chapter describes an analytical workflow for polar lipid determination in hemp. After extraction, lipids are enriched and isolated by graphitized carbon black sorbent, and the isolated lipid is analyzed by liquid chromatography (LC) coupled with high resolution mass spectrometry, leading to identification of many lipid species. We have developed a semi-automated platform using commercially available Lipostar software for lipid identification. Our approach affords the identification of 189 polar lipids in hemp extract, including sulfolipids and phospholipids. The number of the identified lipid species is by far the highest ever reported for Cannabis sativa.
多年来，人们对大麻（Cannabis sativa L.）的化学成分进行了广泛研究，但对其脂类组学却知之甚少。本章描述了一种分析大麻中极性脂质的工作流程。经过提取后，使用石墨化碳黑吸附剂对脂质进行浓缩和分离，然后通过液相色谱（LC）与高分辨率质谱联用对分离出的脂质进行分析，从而鉴定出许多脂质种类。我们使用市售的 Lipostar 软件开发了一个半自动化平台，用于脂质鉴定。我们的方法可以鉴定大麻提取物中的 189 种极性脂质，包括硫脂和磷脂。迄今为止，鉴定出的脂质种类数量是大麻中报道最多的。
