Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China.
Nano Lett. 2021 May 26;21(10):4270-4279. doi: 10.1021/acs.nanolett.1c00408. Epub 2021 May 6.
Engineered bacteria are promising bioagents to synthesize antitumor drugs at tumor sites with the advantages of avoiding drug leakage and degradation during delivery. Here, we report an optically controlled material-assisted microbial system by biosynthesizing gold nanoparticles (AuNPs) on the surface of K3259 () to obtain Bac@Au. Leveraging the dual directional electron transport mechanism of , the hybrid biosystem enhances synthesis of antineoplastic tetrodotoxin (TTX) for a promising antitumor effect. Because of tumor hypoxia-targeting feature of facultative anaerobic , Bac@Au selectively target and colonize at tumor. Upon light irradiation, photoelectrons produced by AuNPs deposited on bacterial surface are transferred into bacterial cytoplasm and participate in accelerated cell metabolism to increase the production of TTX for antitumor therapy. The optically controlled material-assisted microbial system enhances the efficiency of bacterial drug synthesis and provides an antitumor strategy that could broaden conventional therapy boundaries.
工程菌是有前途的生物制剂，可在肿瘤部位合成抗肿瘤药物，具有在输送过程中避免药物泄漏和降解的优势。在这里，我们报告了一种光控材料辅助的微生物系统，通过在 K3259()表面生物合成金纳米粒子 (AuNPs) 来获得 Bac@Au。利用 K3259 的双方向电子传输机制，该混合生物系统增强了抗肿瘤毒素 (TTX) 的合成，具有有前途的抗肿瘤作用。由于兼性厌氧 K3259 的肿瘤缺氧靶向特性，Bac@Au 选择性地靶向并定植于肿瘤部位。在光照射下，沉积在细菌表面的 AuNPs 产生的光电子被转移到细菌细胞质中，并参与加速细胞代谢，以增加 TTX 的产量用于抗肿瘤治疗。光控材料辅助的微生物系统提高了细菌药物合成的效率，并提供了一种抗肿瘤策略，可以拓宽传统治疗的界限。
