Whitford Christopher M, Cruz-Morales Pablo, Keasling Jay D, Weber Tilmann
The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, CA 94608, U.S.A.
Essays Biochem. 2021 Jul 26;65(2):261-275. doi: 10.1042/EBC20200132.
Streptomycetes are producers of a wide range of specialized metabolites of great medicinal and industrial importance, such as antibiotics, antifungals, or pesticides. Having been the drivers of the golden age of antibiotics in the 1950s and 1960s, technological advancements over the last two decades have revealed that very little of their biosynthetic potential has been exploited so far. Given the great need for new antibiotics due to the emerging antimicrobial resistance crisis, as well as the urgent need for sustainable biobased production of complex molecules, there is a great renewed interest in exploring and engineering the biosynthetic potential of streptomycetes. Here, we describe the Design-Build-Test-Learn (DBTL) cycle for metabolic engineering experiments in streptomycetes and how it can be used for the discovery and production of novel specialized metabolites.
链霉菌是多种具有重要医学和工业价值的特殊代谢产物的生产者，如抗生素、抗真菌剂或杀虫剂。在20世纪50年代和60年代曾是抗生素黄金时代的推动者，过去二十年的技术进步表明，到目前为止它们的生物合成潜力很少被开发利用。鉴于抗微生物药物耐药性危机导致对新抗生素的迫切需求，以及对复杂分子进行可持续生物基生产的迫切需要，人们对探索和改造链霉菌的生物合成潜力重新产生了浓厚兴趣。在此，我们描述了链霉菌代谢工程实验的设计-构建-测试-学习（DBTL）循环，以及它如何用于发现和生产新型特殊代谢产物。
