De Sonakshi, Mattanovich Diethard, Ferrer Pau, Gasser Brigitte
Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Austria.
Austrian Centre of Biotechnology (ACIB), Vienna, Austria.
Essays Biochem. 2021 Jul 26;65(2):293-307. doi: 10.1042/EBC20200138.
Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most popular yeast cell factory for the production of heterologous proteins. Strong promoters, stable genetic constructs and a growing collection of freely available strains, tools and protocols have boosted this development equally as thorough genetic and cell biological characterization. This review provides an overview of state-of-the-art tools and techniques for working with P. pastoris, as well as guidelines for the production of recombinant proteins with a focus on small-scale production for biochemical studies and protein characterization. The growing applications of P. pastoris for in vivo biotransformation and metabolic pathway engineering for the production of bulk and specialty chemicals are highlighted as well.
除了面包酵母外，甲基营养型酵母毕赤酵母（也称为巴斯德毕赤酵母）已发展成为生产异源蛋白最受欢迎的酵母细胞工厂。强大的启动子、稳定的遗传构建体以及越来越多可免费获取的菌株、工具和方案推动了这一发展，同样全面的遗传和细胞生物学特征研究也起到了推动作用。本综述概述了用于毕赤酵母研究的最新工具和技术，以及重组蛋白生产指南，重点是用于生化研究和蛋白质表征的小规模生产。文中还强调了毕赤酵母在体内生物转化和代谢途径工程中用于大宗和特种化学品生产的日益广泛的应用。
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