Eckhardt B M, Oeswein J Q, Yeung D A, Milby T D, Bewley T A
Genentech Inc., South San Francisco, California.
J Pharm Sci Technol. 1994 Mar-Apr;48(2):64-70.
An instrumental method to analyze protein solutions for visual appearance is described which is based on spectrophotometric comparison to reference suspensions with varying degrees of turbidity. This method provides a useful substitute for visual inspection of uniform opalescent suspensions in that it is more convenient and less time-consuming and has the potential to be more reproducible, accurate and objective. Established categories of opalescence based on European Pharmacopoeial reference suspensions were determined using turbidity measured as optical density in the 340-360 nm range. A comparison of the mean optical density of a protein sample to those of the reference suspensions allowed a more reproducible assignment of the sample's category of opalescence than that of visual inspection.
描述了一种用于分析蛋白质溶液外观的仪器方法，该方法基于与不同浊度的参考悬浮液进行分光光度比较。这种方法为均匀乳光悬浮液的目视检查提供了一种有用的替代方法，因为它更方便、耗时更少，并且有可能更具可重复性、准确性和客观性。基于欧洲药典参考悬浮液确定的乳光类别是使用在340-360nm范围内作为光密度测量的浊度来确定的。与目视检查相比，将蛋白质样品的平均光密度与参考悬浮液的平均光密度进行比较，可以更可重复地确定样品的乳光类别。
