State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; CAS Center for Excellence in Deep Earth Science, Guangzhou, 510640, China.
J Chromatogr A. 2021 Jun 21;1647:462152. doi: 10.1016/j.chroma.2021.462152. Epub 2021 Apr 20.
Tetrabromobisphenol-A and Tetrabromobisphenol-S (TBBPA/S) and their derivatives have attracted widespread attention owing to their environmental risks and adverse effects on human health. In this study, we developed an analytical method based on ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-MS/MS) for the simultaneous determination of TBBPA/S and their eight derivatives in soil samples. After ultrasonic extraction, TBBPA/S and their derivatives were purified using an LC-Si cartridge with 1 mL acetone and 30 mL dichloromethane/n-hexane (1/1, v/v) as the eluent. In the multiple reaction monitoring (MRM) mode, TBBPA and TBBPS were quantified with electrospray ionization (ESI), whereas their derivatives were quantified with atmospheric pressure chemical ionization (APCI). The proposed method was verified with blank spiking and matrix spiking experiments. All target compounds were recovered at the range of 78-124% and the average recoveries of surrogate standard C-labeled TBBPA were 103% and 99% in spiked blank and soil samples, respectively. The method quantitative limits (MQLs) of TBBPA/S and their derivatives in soil samples ranged from 0.22 to 8.8 pg/g dw. These results indicated that an effective method was provided for the analysis of TBBPA/S and their derivatives in abiotic matrices.
四溴双酚 A 和四溴双酚 S（TBBPA/S）及其衍生物由于其对环境的风险和对人类健康的不良影响而受到广泛关注。在本研究中，我们开发了一种基于超高效液相色谱-串联三重四极杆质谱（UPLC-MS/MS）的分析方法，用于同时测定土壤样品中的 TBBPA/S 及其八种衍生物。经过超声提取后，使用 LC-Si 柱（1 mL 丙酮和 30 mL 二氯甲烷/正己烷（1/1，v/v）作为洗脱液）对 TBBPA/S 及其衍生物进行纯化。在多反应监测（MRM）模式下，用电喷雾电离（ESI）定量测定 TBBPA 和 TBBPS，而用大气压化学电离（APCI）定量测定其衍生物。通过空白加标和基质加标实验对所提出的方法进行了验证。所有目标化合物的回收率在 78-124%范围内，标记的替代标准 C-TBBPA 在加标空白和土壤样品中的平均回收率分别为 103%和 99%。土壤样品中 TBBPA/S 及其衍生物的方法定量限（MQL）范围为 0.22-8.8 pg/g dw。这些结果表明，为分析非生物基质中的 TBBPA/S 及其衍生物提供了一种有效的方法。
