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来自中大西洋海岭南部的深海热液喷口虾类——异尾深海虾(甲壳纲:十足目)的从头转录组组装

De novo transcriptome assembly of the deep-sea hydrothermal vent, shrimp Rimicaris exoculate (Crustacea: Decapoda), from the south Mid-Atlantic Ridge.

作者信息

Lin Wenyang, Shi Hong, Zou Chenchen, Ren Jie, Jian Yiwen, Liu Linmin, Chen Yanjie, Ruan Lingwei

机构信息

State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of Ministry of Natural Resources, Third Institute of Oceanography, Ministry of Natural Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China; School of Life Science, Xiamen University, Xiamen 361005, PR China.

State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of Ministry of Natural Resources, Third Institute of Oceanography, Ministry of Natural Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.

出版信息

Mar Genomics. 2021 Dec;60:100876. doi: 10.1016/j.margen.2021.100876. Epub 2021 May 3.

DOI:10.1016/j.margen.2021.100876
PMID:33958310
Abstract

The deep-sea hydrothermal vent is a special ecosystem, which is different from terrestrial or coastal ecosystems. Rimicaris exoculata, which adapts well to several deep-sea hydrothermal vent environments, is the ideal model for studying hydrothermal vent fauna. In the present study, we obtained R. exoculata from a newly found hydrothermal vent in the south Mid-Atlantic Ridge, and the Illumina next-generation sequencing and de novo assembly were performed by Beijing Genomics Institution. A total of 17,258 annotated Unigenes were obtained. Several Unigenes associated with sulfide metabolism, which might contribute to well adaptation to high concentration of sulfide for R. exoculata, were annotated. This study is the first report on the high-throughput sequencing of R. exoculata. Our data can allow for further studies on the ability of R. exoculata adaptation to harsh conditions and provide abundant gene resources for research and development.

摘要

深海热液喷口是一种特殊的生态系统,与陆地或沿海生态系统不同。适应多种深海热液喷口环境的长腕铠甲虾是研究热液喷口动物群的理想模型。在本研究中,我们从新发现的中大西洋海岭南部热液喷口采集了长腕铠甲虾,并由北京基因组研究所进行了Illumina新一代测序和从头组装。共获得17258个注释单基因。注释了几个与硫化物代谢相关的单基因,这可能有助于长腕铠甲虾对高浓度硫化物的良好适应。本研究是关于长腕铠甲虾高通量测序的首次报道。我们的数据可以为进一步研究长腕铠甲虾适应恶劣条件的能力提供支持,并为研发提供丰富的基因资源。

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